Affiliations: Dipartimento di Biochimica "G. Moruzzi",
Università di Bologna, Italy
Note: [] Address for correspondence: Professor Laura Landi, Dipartimento
di Biochimica "G. Moruzzi"-Università degli Studi di Bologna, Via Irnerio,
48 (40126) Bologna, Italy. Tel.: +39 0512091209; Fax: +39 051351234; E-mail:
[email protected]
Abstract: Reactive oxygen species (ROS) has been increasingly recognised as
intracellular messengers in signal transduction following receptor activation
by a variety of bioactive peptides including growth factors, cytokines and
hormones. In this study ROS production and glucose transport activity were
evaluated in the growth factor dependent M07e cells and in B1647 cells, not
requiring additional hematopoietic cytokines for growth: the aim was to
investigate whether ROS could be involved in the regulation of Glut1-mediated
glucose uptake in both cell lines. The effect of the synthetic superoxide and
hydrogen peroxide scavenger EUK-134 on DOG uptake activity and intracellular
ROS formation supports the concept of reactive oxygen species as signalling
molecules. In order to investigate ROS generation sources, diphenyleneiodonium,
an inhibitor of flavoprotein centres and apocynin, an inhibitor of NAD(P)H
oxidase, were used: they inhibit both ROS production and glucose uptake
activation. All these data support the hypothesis that ROS can contribute to
the regulation of glucose transport, not only in M07e cells but also in B1647
cells; we could speculate that one possible source of ROS, linked somehow with
Glut1 activity, can be a NAD(P)H oxidase similar to that one present in
phagocytic cells.
