Measurement of isoflavones using liquid chromatography with multi-channel coulometric electrochemical detection

Issue title: The Proceedings of the 3rd International Conference on Food Factors (ICoFF 03)

Article type: Research Article

Authors: Ouchi, Kazuyuki^; | Gamache, Paul | Acworth, Ian | Watanabe, Shaw

Affiliations: Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-0054, Japan | MC Medical, Inc., 7-5-25 Nishishinjuku, Shinjuku-ku, Tokyo 160-8355, Japan | ESA, Inc., 22 Alpha Road, Chelmsford, MA 01824-4171, USA

Note: [] Corresponding author. E-mail: [email protected]

Abstract: We evaluated the use of HPLC multi-channel coulometric electrochemical detection (HPLC-ECD) for the routine measurement of phytochemicals by analyzing standards and urine samples. Compounds were separated chromatographically and electrochemically with high sensitivity (10 ng/ml of isoflavones in urine). HPLC-ECD will enable better investigation into the relationship between lifestyle and the effects of phytochemicals on humans.

Journal: BioFactors, vol. 22, no. 1-4, pp. 353-356, 2004