Comparison of an oxidative stress biomarker "urinary8-hydroxy-2'-deoxyguanosine," between smokers and non-smokers

Issue title: The Proceedings of the 3rd International Conference on Food Factors (ICoFF 03)

Article type: Research Article

Authors: Kanaya, Setsuko | Ikeya, Masae | Yamamoto, Kanako | Moriya, Takayo | Furuhashi, Keiko | Sonoda, Makiko | Goto, Koichi | Ochi, Hirotomo

Affiliations: Department of Clinical Nutrition, Seirei Mikatabara Hospital, Japan | Internal medicine, Seirei Mikatabara Hospital, Japan | Japan Institute for the Control of Aging (JaiCA), Japan

Note: [] Corresponding author. Fax: +81 43 486 6916; E-mail: [email protected]

Abstract: A great deal of effort has been made on the effect of oxidative stress for smokers. What seems to be lacking, however, is its evidence. Analyzing 1076 participants (age 35.9 ± 12.9, urinary8-OHdG Mean ± S.D., 11.4 ± 6.7, n = 1076), our study found the significant increase in a biomarker of DNA damage urinary 8-OHdG/creatinine among smokers (7.75 ± 2.8 ng/ml × CRE (n = 154) and 7.36 ± 2.5 ng/ml × CRE (n = 627) (p < 0.05), Relative Risk = 2.9 (1.4–6.2) sex and age ± 2 matching 105 male smokers and non-smokers. There was no significance on the comparison between female smokers and non-smokers. Smokers have significantly decreased serum alpha-tocopherol (1012 ± 455, 1152 ± 857, p < 0.03). The amount of serum ascorbate did not change. Smokers lowered serum HDL-cholesterol compared to non-smokers (59.3 ± 11.8, 63.9 ± 13.3, p < 0.05). The result of oxidative stress profile (OSP) also indicated that the increase of oxidative stress to smokers (p < 0.05). The calculated value of oxygen radical absorbance capacity (ORAC) of the meal for subjects was 1600 ORAC units.

Keywords: smokers, urinary8-OHdG (urinary8-hydroxy-2'-deoxyguanosine), oxidative stress profile (OSP), oxygen radical absorbance capacity (ORAC)

Journal: BioFactors, vol. 22, no. 1-4, pp. 255-258, 2004