Affiliations: National Agricultural Research Center for Tohoku
Region, Fukushima 960-2156, Japan | Food and Biodynamic Chemistry Laboratory, Graduate
School of Life Science and Agriculture, Tohoku University, Sendai 981-8555,
Japan | Fukushima Technology Center Aizuwakamatsu Technical
Support Center, Aizuwakamatsu 965-0006, Japan | National Food Research Institute, Tsukuba 305-8642,
Japan
Note: [] Address for correspondence: Toshiyuki Kimura, Laboratory of
Post-harvest Crop Management, National Agricultural Research Center for Tohoku
Region, 50 Harajukuminami, Arai, Fukushima-city, Fukushima, 960-2156, Japan.
Tel.: +81 24 593 6178; Fax: +81 24 593 2155; E-mail: [email protected]
Abstract: A simple and rapid method for determining 1-deoxynojirimycin (DNJ),
a potent glucosidase inihibitor present in mulberry leaves (Morus alba
and Morus bombysis), by high performance liquid chromatography coupled to
an evaporative light scattering detector (ELSD) has been developed. DNJ was
separated from extract of mulberry leaves on TSK gel Amide-80 column, which is
a representative column for hydrophilic interaction chromatography. During post
column detection, DNJ was detected by ELSD and concurrently identified by mass
spectrometry. The detection limit was 100 ng. This method is sufficiently
sensitive for determining DNJ in mulberry leaves and other related
products.
Keywords: 1-Deoxynojirimycin, evaporative light scattering detector, mulberry leaves, morus alba
Journal: BioFactors, vol. 22, no. 1-4, pp. 341-345, 2004
