Quantitative determination of urinary 8-hydroxy-2'-deoxyguanosine level in healthy Japanese volunteers

Issue title: The Proceedings of the 3rd International Conference on Food Factors (ICoFF 03)

Article type: Research Article

Authors: Miwa, Misao | Matsumaru, Hiroyuki | Akimoto, Yukari | Naito, Shigehiro | Ochi, Hirotomo

Affiliations: National Food Research Institute, Tsukuba 305-8642, Japan | Japan Institute for the Control of Aging, Fukuroi, Shizuoka 437-0122, Japan

Note: [] Corresponding author. Tel.: +81 3 5477 2576; Fax: +81 3 5477 2576; E-mail: [email protected]

Abstract: 8-hydroxy-2'-deoxyguanosine (8-OHdG), as a measure of oxidative stress, was measured in healthy Japanese volunteers using an ELISA (New 8-OHdG Check®, JICA). Analysis of daytime spot urine of 83 healthy male subjects and smoking habit, exercise and age revealed significant correlation only between the urinary level of 8-OHdG and age. As the inter-individual variation of 8-OHdG of the daytime spot urine was relatively high, we next determined inter-and intra-individual variation of 5 healthy volunteers. The levels of 8-OHdG/creatinine in morning spot urine significantly correlated with 8-OHdG levels in 24-h pool urine. Thus, a morning spot urine sample can be used for the measurement of 8-OHdG instead of inconvenient 24-h sampling.

Keywords: 8-hydroxy-2'-deoxyguanosine

Journal: BioFactors, vol. 22, no. 1-4, pp. 249-253, 2004