Affiliations: R & D Center, Alcohol Enterprise Head Office, New
Energy and Industrial Technology Development Organization (NEDO), 5-1,
Inagehigashi 4-chome, Inage-ku, Chiba-shi, Chiba, 263-0031, Japan | Mercian Cleantec Co., 9-1, Johnan 4-chome,
Fujisawa-shi, Kanagawa, 251-0057, Japan
Abstract: Lactic acid bacteria were isolated from an alcohol fermentation
broth, and the activity as a probiotic was examined using pathogenic E. coli.
Thirty-six strains exhibiting good growth were isolated in the medium of
concentrated mush which was a residue resulted in the alcohol distillation
process. One of these strains, Lactobacillus paracasei subsp. paracasei I-5,
could be grown in the medium containing 8 vol% ethanol and at 45°C. The
characteristics were different from the type strain, L. paracasei subsp.
paracasei NBRC 15889™. L. paracasei I-5 showed an excellent growth in the
concentrated mush, which just diluted two-fold and adjusted the pH. ICR mice
were fed with a standard germ-free feed (CMF) and the strain I-5 (7 ×
10^9 cells/day) was orally administrated for 11 days prior
to the intraperitoneal challenge with pathogenic E. coli Juhl. After the
challenge, mice administrated the strain I-5 exhibited a high survival rate and
survival extension days (p < 0.01) compared with the control. The results
suggested that the strain might enhance the animal resistance against microbial
pathogens. Neonatal diarrhea caused by E. coli is a serious disease in
calf breeding. The strain might be practically valuable to prevent diarrhea in
calves.
Keywords: lactic acid bacteria, Lactobacillus paracasei, pathogenic E. coli
Journal: BioFactors, vol. 21, no. 1-4, pp. 155-158, 2004
