Cytoplasmic fraction of Lactococcus lactis ssp. lactis induces apoptosis in SNU-1 stomach adenocarcinoma cells

Issue title: The Proceedings of the 3rd International Conference on Food Factors (ICoFF 03)

Article type: Research Article

Authors: Kim, Seo Young | Lee, Ki Won | Kim, Ji Yeon | Lee, Hyong Joo

Affiliations: Department of Food Science and Technology, School of Agricultural Biotechnology, Seoul National University, Seoul 151-741, South Korea

Note: [] Corresponding author. Tel.: +82 2 880 4853; Fax: +82 2 873 5095; E-mail: [email protected]

Abstract: Lactic acid bacteria are known to have antitumor activity, but the underlying mechanisms remain unclear. Recently we showed that a cytoplasmic fraction – but not peptidoglycan – of Lactococcus lactis ssp. lactis (L.lac CF) had strong antiproliferative activity on SNU-1 human stomach adenocarcinoma cells. The present study investigated whether the antiproliferative activity of L.lac CF on SNU-1 is linked to the induction of apoptosis. Treatment of L.lac CF inhibited the proliferation of SNU-1 cells in a dose- and time-dependent manner. Furthermore, treatment of the cells with 50 μg/ml and 100 μg/ml L.lac CF resulted in DNA fragmentation and chromatin condensation, respectively. The results indicate that the inhibitory effect of L.lac CF on SNU-1 cell growth is mainly attributable to the induction of apoptosis.

Keywords: Lactococcus lactis ssp. lactis, cytoplasmic fraction, SNU-1 human stomach adenocarcinoma cell line, apoptosis

Journal: BioFactors, vol. 22, no. 1-4, pp. 119-122, 2004