Production of nitric oxide, tumor necrosis factor-α and
interleukin-6 by RAW264.7 macrophage cells treated with lactic acid bacteria
isolated from kimchi
Issue title: The Proceedings of the 3rd International
Conference on Food Factors (ICoFF 03)
Affiliations: Department of Food Science and Technology, School of
Agricultural Biotechnology, Seoul National University, Seoul 151-742, South
Korea
Note: [] Address for correspondence: Professor Hyong Joo Lee Department
of Food Science and Technology, Seoul National University, Shinlim-dong,
Kwanak-ku, Seoul 151-742, South Korea. Tel.: +82 2 880 4853; Fax: +82 2 873
5095; E-mail: [email protected]
Abstract: The present study investigated immunopotentiating activities of
lactic acid bacteria isolated from kimchi (KLAB) in vitro. A RAW264.7
macrophage cell line was stimulated with four strains of KLAB and two strains
of bifidobacteria, and then the production of nitric oxide (NO) and cytokines
– tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6)
– was determined. Lactobacillus plantarum, a lactic acid bacteria
involved in the latent fermentation of kimchi, was the most effective for the
generation of NO, TNF-α, and IL-6 in macrophage. All strains generated
NO, while for the TNF-α and IL-6, only L. plantarum had significant
activity. Our results indicate that L. plantarum plays an important role in the
immunopotentiating activity of kimchi.
