Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Issue title: The Proceedings of the 3rd International Conference on Food Factors (ICoFF 03)
Article type: Research Article
Authors: Baker, Mark A. | Ly, Jennifer D. | Lawen, Alfons
Affiliations: Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, P.O. Box 13D, 100 Wellington Road, Melbourne, Victoria 3800, Australia | ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Science, University of Newcastle, NSW, Australia
Note: [] Corresponding author. Tel.: +61 3 9905 3711; Fax: +61 3 9905 3726; E-mail: [email protected]
Abstract: We have recently demonstrated that voltage dependent anion selective channel~1 (porin, isoform 1) can function as a transplasma membrane NADH:ferricyanide-reductase. However, both the specific redox characteristics and the mechanism of electron transport in this enzyme presently remain unclear. Here we demonstrate that the redox capability of porin 1 is specific for ferricyanide as this same enzyme cannot reduce DCIP or cytochrome c in vitro. Furthermore, NADH-dependent ferricyanide reduction associated with VDAC1 is not sensitive to the anion channel inhibitors DIDS and dextran sulfate. However, this activity can be inhibited by thiol chelators, suggesting that at least one of the two cysteine groups present in VDAC1 are critical for electron transfer. We propose a model on how electron transport may occur in VDAC1.
Keywords: transplasma membrane electron transport, NADH, VDAC1, ferricyanide reductase
Journal: BioFactors, vol. 21, no. 1-4, pp. 215-221, 2004
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
[email protected]
For editorial issues, like the status of your submitted paper or proposals, write to [email protected]
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
[email protected]
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office [email protected]
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
[email protected]
For editorial issues, like the status of your submitted paper or proposals, write to [email protected]
如果您在出版方面需要帮助或有任何建, 件至: [email protected]