Evidence that selenium deficiency results in the cytoplasmic decay of GPx1 mRNA dependent on pre-mRNA splicing proteins bound to the mRNA exon-exon junction

Article type: Research Article

Authors: Maquat, Lynne E.

Affiliations: Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, 601 Elmwood Avenue, Box 712 Rochester, NY 14642, USA. Tel.: +1 716 273 5640; Fax: +1 716 271 2683; E-mail: [email protected]

Abstract: Studies of transcripts for the selenoprotein glutathione peroxidase 1 (GPx1) have provided remarkable evidence for the coupling of pre-mRNA splicing in the nucleus and mRNA translation in the cytoplasm. Such evidence derives from the initial finding that GPx1 mRNA is a natural substrate of nonsense-mediated decay. Here, recent work on GPx1 RNA metabolism is reviewed and future directions of study are defined.

Keywords: glutathione peroxidase 1 mRNA, nonsense-mediated decay, pre-mRNA splicing, mRNP structure, Upf proteins

Journal: BioFactors, vol. 14, no. 1-4, pp. 37-42, 2001