Generation of lipid peroxyl radicals from edible oils and their biological activities: A need for consideration for anti-radical components and purification processing

Article type: Research Article

Authors: Ayako Kanazawa, | Tomohiro Sawa, | Takaaki Akaike, | Shigeru Morimura, | Kenji Kida, | Hiroshi Maeda,

Affiliations: Faculty of Education, Kumamoto University, Kurokami, Kumamoto 860-0811, Japan. Tel.: +81 96 373 5098; Fax: +81 96 362 8362 | Department of Microbiology, School of Medicine, Kumamoto University, Honjo 2-2-1, Kumamoto 860-0811, Japan. Tel.: +81 96 373 5098; Fax: +81 96 362 8362; E-mail: [email protected] | Faculty of Engineering, Kumamoto University, 1, 3 Kurokami, Kumamoto 860-0811, Japan. Tel.: +81 96 373 5098; Fax: +81 96 362 8362

Abstract: Lipid hydroperoxides (LOOH or oxidized oils) are known as unfavorable food components. Molecular details of the fate and mechanisms of LOOH to exert adverse effects in vivo are, however, little understood. In the present study, we demonstrated that LOOH generated alkylperoxyl radical (LOO^{\cdot }) after reaction with various heme compounds such as myoglobin, cytochrome c, hemin, hematin, etc., but little formation of other radical species was noticed such as L^{\cdot} or LO^{\cdot}. It was also shown that LOO^{\cdot } thus formed exhibits cytotoxicity and caused DNA damages including strand breakage and abasic site formation. This highly toxic LOO^{\cdot } is effectively scavenged by hot water extracts of vegetable (soup), flavonoids, polyphenols as well as tocopherols. Another important finding is that crude vegetable oils are rich in potent-LOO^{\cdot } scavenging activity, which exhibits potent anti-oxidant activity as well; whereas highly purified oils are scanty in such components and LOO^{\cdot } scavenging activity. These findings imply that a considerate processing in the refining of oils should be needed to retain such potent endogenous anti-oxidative radical scavenging-components.

Journal: BioFactors, vol. 13, no. 1-4, pp. 187-193, 2000