Affiliations: Laboratory of Food and Biodynamics, Nagoya University
Graduate School of Bioagricultural Sciences, Nagoya 464-8601, Japan | Division of Biomodeling, Nagoya University Graduate
School of Bioagricultural Sciences, Nagoya 464-8601, Japan
Note: [] Laboratory of Food Chemistry, Department of Nutrition and Food
Science, Ochanomizu University, Otsuka 2-1-1, Bunkyo-ku, Tokyo 112-8610, Japan.
Tel./Fax: +81 3 5978 5757; E-mail: [email protected]
Abstract: 6-Methylsulfinylhexyl isothiocyanate (MS-ITC) was isolated from
wasabi ({\it Wasabia japonica}, Japanese domestic horseradish) as a potential
inhibitor of human platelet aggregation {\it in vitro} through our extensive
screening of vegetables and fruits. In the course of another screening for the
induction of glutathione S-transferase (GST) activity in
RL34 cells, MS-ITC was inadvertently isolated from wasabi as a potential
inducer of GST. MS-ITC administered to rats or mice also showed both activities
{\it in vivo}. As a result from elucidation of the platelet aggregation
inhibition and the GST induction mechanisms of MS-ITC, the isothiocyanate
moiety of MS-ITC plays an important role for antiplatelet and anticancer
activities because of its highly reactivity with sulfhydryl (-SH) groups in
biomolecules (GSH, cysteine residue in a certain protein, etc.).
Keywords: isothiocyanate, platelet aggregation, phase II enzyme, glutathione [TeX:] s-transferase, cancer chemoprevention
Journal: BioFactors, vol. 13, no. 1-4, pp. 271-276, 2000
