Journal of Berry Research - Volume 9, issue 2

Authors: Jaimes-Gualdrón, Tania Rocío | Alzate Ceballos, Johanna Andrea | Rojano, Benjamın Alberto

Article Type: Research Article

Abstract: BACKGROUND: Oxidation is a deteriorating process in foods that affects sensorial quality and nutritional value. As a result, there has been a tendency to develop and consume functional antioxidant foods which delay oxidative processes. Anthocyanins are polyphenols which have been associated with high antioxidant activity. OBJECTIVE: To study the protection effect of corozo and grape extract with high anthocyanin content, on the oxidation time of yogurt. METHODS: Colorimetric and fluorimetric assays were used to determine anthocyanin content and antioxidant capacity in the extracts over time. For the yogurt, total phenol content was evaluated and antioxidant capacity …by ABTS and ORAC. Also, the pH and color parameters (CIELAB - L*a*b*) were determined for the extracts and the yogurt. Finally, the oxidation of lipids and proteins in the yogurt were determined. RESULTS: A mixture of extracts from corozo and grape had good oxidative stability during the first month of study. There was also protection from oxidation in proteins and lipids during the 21 days of study in a yogurt prepared with the mixture of extracts. CONCLUSIONS: The extracts prepared from corozo and grape are adequate to be used as protectors of oxidation of macromolecules in yogurt. Show more

Keywords: Antioxidant, anthocyanins, yogurt, stability

DOI: 10.3233/JBR-180327

Citation: Journal of Berry Research, vol. 9, no. 2, pp. 141-153, 2019

Authors: Hernández-Bautista, Aurelio | Lobato-Ortiz, Ricardo | García-Zavala, J. Jesús | Mejía Contreras, José Apolinar | Chávez-Servia, José Luis | Rodríguez, Mario Rocandio | Cruz-Izquierdo, Serafin | García Velázquez, José Armando

Article Type: Research Article

Abstract: BACKGROUND: The worldwide tendency for growing raspberries in different climatic environments demands the need of development new cultivars with good adaptability. OBJECTIVE: The aim of the present study was to study the adaptability of red raspberry selections for tropical conditions. RESULTS: Days to flowering, days from flowering to first pick, days to first pick, and days from transplanting to last pick were from 135 to 183 days, 22 to 49 days, 163 to 222.3 days, 250.0 to 299.5 days, respectively. Harvest period varied among genotypes, and the genotypes S.39 and S.27 had the …longest harvest period and the highest marketable yields, evidencing their good adaptability to tropical conditions. Berry weight varied from 2.74 to 5.37 g, suggesting that the studied selections produced fruits with small and intermedium size. In addition, these results indicated that such genotypes might be suitable for fruit exportation to different international markets. CONCLUSION: The present study demonstrated the adaptability of certain genotypes such as S.39 and S.27 at a tropical environment, which might be a good option for raspberry growers producing for fresh consumption. Show more

Keywords: Rubus idaeus , agronomic performance, berry size, raspberry breeding.

DOI: 10.3233/JBR-180328

Citation: Journal of Berry Research, vol. 9, no. 2, pp. 155-163, 2019

Authors: Sánchez, S. | Grez, J. | Contreras, E. | Gil, P.M. | Gambardella, M.

Article Type: Research Article

Abstract: BACKGROUND: Charcoal rot of strawberry (Macrophomina phaseolina ) is an emerging disease difficult to manage, a desirable alternative is the use of resistant cultivars. However, little is known regarding the reaction of cultivars to the pathogen under water stress conditions. OBJECTIVE: The aims of this work were to study the effect of water stress on the physiology of four strawberry cultivars during the infection, and to determine the relationship between water stress and cultivar susceptibility. METHODS: Healthy and inoculated plants of ‘Monterey’, ‘Albion’, ‘Camarosa’ and ‘Sabrina’ were maintained under no irrigation and full …irrigation regimes, in greenhouse conditions. Stem water potential (SWP) and stomatal conductance (gs) were evaluated. The disease severity was recorded weekly for seven weeks. RESULTS: The disease detrimentally affected the water relations in ‘Sabrina’, ‘Albion’ and ‘Monterey’. A significant correlation was detected between the evaluated parameters and the disease severity. The disease severity increases in plants with no irrigation, regardless of cultivar. CONCLUSIONS: Our results show that the infection caused by M. phaseolina increases the negative effects of water stress, depending on the genotype, and that the cultivars that were able to maintain more stable water relations respond better to the disease. Show more

Keywords: Crown and root rot, water restriction, biotic stress, abiotic stress, biotic-abiotic stresses combination

DOI: 10.3233/JBR-180329

Citation: Journal of Berry Research, vol. 9, no. 2, pp. 165-177, 2019

Authors: Khan, Rao Sanaullah | Asghar, Waqas | Khalid, Nauman | Nazir, Wahab | Farooq, Muhammad | Ahmed, Iftikhar | Syed, Qamar Abbas

Article Type: Research Article

Abstract: Fruit berries are one of the most effective source of bioactive food ingredients with multiple health benefits when consumed regularly. Phalsa fruit (Grewia asiatica L.) a native to the Himalayan region grows equally well in tropical areas of the world yet unexplored with regards to its immense nutritional benefits. The phalsa seed, fruit, and pulp contain numerous functional phytochemicals that can be used to treat various diseases, and have be found to be highly effective in improving respiratory and cardiac functioning. Its cultivation has been limited to subsistence cultivation and it is sold in the form of raw fruit …mostly. There are certain challenges as regards to its perishable nature of the berry fruit, and the optimization of the crop yield. Therefore, this comprehensive review is designed to highlight its economic and nutritional potential for the food and beverage industry as an effective source of bioactive functional food/beverage ingredients. Further potential area of research and developments have been identified for the subsequent authentication of health effects of phalsa berry fruit. Moreover, issues related to value addition in food product development have been explained along with proposed solutions. Show more

Keywords: Phalsa, bioactive compounds, health benefits, processing challenges, anthocyanins

DOI: 10.3233/JBR-180332

Citation: Journal of Berry Research, vol. 9, no. 2, pp. 179-193, 2019

Authors: Orjuela-Palacio, Juliana M. | Zamora, Maria Clara | Lanari, Maria Cecilia

Article Type: Research Article

Abstract: BACKGROUND: Yerba-mate (YM) and blackcurrant (BC) are rich in polyphenols and anthocyanins with proven health benefits therefore they could be used in functional beverage preparation. OBJECTIVE: To (a) develop a palatable powdered beverage with high physicochemical and nutritional quality using YM/BC, (b) determine the effect of in-vitro gastrointestinal digestion on antioxidant content and activity and (c) evaluate beverage’s acceptance temporal changes and dynamic profile during multiple intakes. METHODS: We determined powder’s water-activity (aw), moisture content, flowability, micro-morphology, color, cold water solubility, glass-transition temperature (Tg), total polyphenols (TP)/anthocyanins (MAC) and ascorbic-acid contents, antiradical activity (DPPH). …RESULTS: Powder’s aw (0.089) ensured biochemical/microbiological stability. Tg(55.64°C) indicated that YM/BC’s vacuum-storage at 20–25 °C was possible without losing glassy state. Powder’s cold water’s solubility was 90%. 1 L (60 g/L) provided 556.8 mg TP/198.11 mg monomeric-anthocyanins. Ascorbic- acid dose was half the recommended daily intake. In-vitro gastrointestinal digestion reduced total polyphenols/monomeric-anthocyanins 59–70 %, but antiradical activity losses were 9%. Overall-acceptance and Time-Intensity curves results were in the Hedonic scale’s “Like moderately to like very much” zone. Berries/Sweet were the only dominant attributes Astringency accumulative effects not detected. CONCLUSIONS: The YM/BC powder had good physical/biochemical/microbiological stability under industrial storage conditions and high antiradical activity after simulated gastrointestinal digestion. Sensory scores suggested that consumer’s acceptance could be high. Show more

Keywords: Yerba mate, blackcurrant, antioxidant activity, dynamic sensory methods, in vitro gastrointestinal digestion

DOI: 10.3233/JBR-180342

Citation: Journal of Berry Research, vol. 9, no. 2, pp. 195-208, 2019

Authors: Chater, John M. | Mathon, Caroline | Larive, Cynthia K. | Merhaut, Donald J. | Tinoco, Luzineide W. | Mauk, Peggy A. | Jia, Zhenyu | Preece, John E.

Article Type: Research Article

Abstract: BACKGROUND: Pomegranate (Punica granatum L.) juice is a highly-valued beverage that has been demonstrated to have large quantities of polyphenolic compounds with powerful antioxidant properties. Currently there is limited information related to other components of the juices of USDA germplasm. OBJECTIVE: Preselected pomegranate germplasm was evaluated to identify unique cultivars with consumer-friendly traits and to select candidates that meet or exceed juice quality parameters of ‘Wonderful’ juice. METHODS Juices from 14 cultivars and commercial pomegranate juice were analyzed using a variety of methods. Juice quality was determined by measuring pH, titratable acidity, citrate, malate, total …soluble solids, maturity index, glucose, fructose, γ -Aminobutyric acid (GABA), antioxidant activity, total phenolics, potassium, glutamate, glutamine, and ethanol. RESULTS Candidate cultivars meeting ‘Wonderful’ juice quality specifications and nutritional profile were identified as were candidates with desirable traits not seen in ‘Wonderful’. There were significant differences among cultivars in pH, titratable acidity, citrate, malate, total soluble solids, maturity index, glucose, fructose, GABA, potassium, glutamate, glutamine, and ethanol. There was no evidence for differences in antioxidant activity and total phenolics of juices among cultivars. CONCLUSIONS: Pomegranate cultivars other than ‘Wonderful’ could provide a broader palette of flavors for the consumer, but more research is needed to determine commercial potential of USDA germplasm. Show more

Keywords: Amino acids, DPPH, germplasm, lythraceae, potassium, wonderful, pomegranate

DOI: 10.3233/JBR-180344

Citation: Journal of Berry Research, vol. 9, no. 2, pp. 209-225, 2019

Authors: Perpetuini, G. | Donati, I. | Cellini, A. | Orrú, L. | Giongo, L. | Farneti, B. | Spinelli, F.

Article Type: Research Article

Abstract: BACKGROUND: Raspberry breeding programs allowed the development of highly yielding cultivars, but often resulted in a reduction of plant plasticity and resistance to abiotic and biotic stresses. The epiphytic bacterial community on leaves and fruit may play a crucial role in overcoming these shortcomings, influencing host performance and health status. OBJECTIVE: The bacterial community associated to two red-fleshed (“Imara” and “Regina”) and one white-fleshed (“Anne”) raspberry varieties was described. The bacterial community was functionally characterized to identify strains with plant growth promoting or plant protection traits. METHODS: Microbial community was assessed using both culture-independent and -dependent …methods. Strains were tested for production of acetoin, siderophores, indoleacetic acid and ammonia, ACC deaminase activity, biofilm formation, biological control of Erwinia amylovora , Botrytis cinerea or Drosophila suzukii . RESULTS: The fruit bacterial community clearly differed between red-and white-fleshed raspberry. Thirteen isolates produced plant growth and resistance promoting substances, while twelve bacterial isolates were able to manipulate either auxin or ethylene metabolism. Five strains inhibited the growth of Erwinia amylovora and Botrytis cinerea , and one increased Drososphila suzukii mortality. CONCLUSIONS: This study offers new insights for the biotechnological exploitation of bacteria isolated from raspberry. Show more

Keywords: Rubus idaeus, metagenome, plant growth promoting, biological control, Erwinia amylovora, Drosophila suzukii, Botrytis cinerea, IAA, acetoin, ACC deaminase, siderophore

DOI: 10.3233/JBR-180340

Citation: Journal of Berry Research, vol. 9, no. 2, pp. 227-247, 2019

Authors: Balducci, Francesca | Capriotti, Luca | Mazzoni, Luca | Medori, Irene | Albanesi, Andrea | Giovanni , Borraccini | Giampieri, Francesca | Mezzetti, Bruno | Capocasa, Franco

Article Type: Research Article

Abstract: BACKGROUND: New sweet cherries rootstocks and cultivars can be valorized on the market if they express improved plant yield efficiency and fruit quality, and also including highest content of antioxidant compounds (mainly anthocyanins but also significant amounts of phenolic acids and flavonols). OBJECTIVE: The goal of this study was to compare the effect of 10 new rootstock genotypes, now available for cherry, on plant growth and fruit quality of the cultivar “Sweetheart”, a very late auto-fertile sweet cherry cultivar. METHODS: Plants vegetative and productive parameters, as well as fruit sensorial quality, were measured during a 5-year …cycle (2011–2015), while fruit nutritional quality was investigated for the last 3 years (2013–2015). RESULTS: For the agronomical traits, the vigorous rootstock Adara/Major and intermediate Gisela 6 stands out for the plant yield. From the qualitative point of view, the intermediate MaxMa 14 and Gisela 6, and the dwarf Gisela 5, differed for reduced fruit size but with optimal sugar/acid ratio. The higher fruit nutritional value were detected for “Sweetheart” trees grafted on Adara/Major (vigorous), the best MaxMa 14 (weaker) and Gisela 5 (dwarfing) rootstocks. CONCLUSIONS: There is not a univocal combination between the scion and rootstock equally valid for all the considered parameters. The rootstock capable of ensuring good yields, sensorial and nutritional quality is Adara/Major. From the productive and qualitative point of view, Gisela 6 is well adapted to these conditions, while MaxMa 14 rootstock stands out for nutritional quality, although induces a high reduction of vigor, poor vegetative renewal and low average fruit weight. Show more

Keywords: Scion, sweetheart, plant yield, sensorial quality, nutritional quality

DOI: 10.3233/JBR-180345

Citation: Journal of Berry Research, vol. 9, no. 2, pp. 249-265, 2019

Authors: Muceniece, Ruta | Klavins, Linards | Kviesis, Jorens | Jekabsons, Kaspars | Rembergs, Reinis | Saleniece, Kristine | Dzirkale, Zane | Saulite, Liga | Riekstina, Una | Klavins, Maris

Article Type: Research Article

Abstract: BACKGROUND: Berry pomaces contain biologically active substances and therefore may have considerable potential applications in food and pharmaceutical industries. However, there is a lack of studies on extracting these valuable products from berry pomaces. OBJECTIVE: This study aims to evaluate major constituents of five Vaccinium spp. berry pomace extracts and investigate their antioxidant, hypoglycaemic and hepatoprotective properties. METHODS: Total anthocyanin, procyanidin and polyphenol contents in berry residues were measured by spectrophotometric pH-differential, 4-dimethylaminocinnamaldehyde and Folin–Ciocalteu method, respectively. Identification and quantification of separate compounds were assessed by ultra-performance liquid chromatography and liquid chromatography-time-of-flight-masspectroscopy. Anti-hyperglycaemic properties of …pomace extracts were evaluated based on inhibition of α -amylase and α -glucosidase activity. Extract hepatoprotective effects were estimated based on increased human hepatic cell line HepG2 viability in oxidative stress conditions. RESULTS: The extracts possessed strong antioxidant effects and efficacy for inhibiting the activities of enzymes involved in carbohydrate digestion and for protecting hepatic cells from oxidative damage. CONCLUSIONS: Hepatoprotective and hypoglycaemic properties of berry pomace extracts may be due to the presence of active polyphenols and their synergistic effects. Our results support the use of berry pomaces for designing standardised formulations for the prevention of chronic diseases associated with oxidative stress. Show more

Keywords: Vaccinium spp. berry extracts, antioxidative properties, hepatoprotection, hypoglycaemic effect, α-amylase, α-glucosidase

DOI: 10.3233/JBR-180351

Citation: Journal of Berry Research, vol. 9, no. 2, pp. 267-282, 2019

Authors: Soto, Marvin | Pérez, Ana M. | Cerdas, María del Milagro | Vaillant, Fabrice | Acosta, Óscar

Article Type: Research Article

Abstract: BACKGROUND: Blackberry fruits have recently gained attention because of their considerable amounts of polyphenols. OBJETIVE: The aim of this study is to compare the physicochemical composition and contents of the main polyphenolic compounds of nine tropical highland blackberry genotypes grown in Costa Rica, and to evaluate the effect of environmental conditions and farming practices on physicochemical composition of fruits of ‘Wine red thorn’, which is the most widely grown blackberry genotype in Costa Rica. METHODS: Nine blackberry genotypes were harvested at fully ripe stage. Environmental conditions (geographic location and harvest season) and farming practices (organic and …conventional techniques) were evaluated to determine their effects on physicochemical composition and polyphenolic compounds of ‘Wine red thorn’ blackberries. Anthocyanins and ellagitannins contents were determined by HPLC-DAD. RESULTS: The tropical highland blackberries contained appreciable levels of polyphenols. Anthocyanin content ranged from 2.9±0.1 to 12±1 g cyanidin-3-glucoside equivalents/kg dry weight (DW); ellagitannin content ranged from 9.3±0.8 to 47±3 g ellagic acid equivalents/kg DW. The influence of environmental conditions and farming practices on physicochemical characteristics of ‘Wine red thorn’ blackberries was significant (p  < 0.05). Blackberries harvested during the dry season had higher contents of sugars and anthocyanins than blackberries harvested during the rainy season. Organic blackberries had higher levels of ellagitannins than conventionally grown fruits. CONCLUSIONS: These data prove that Costa Rican blackberries are a good source of polyphenolic compounds, especially ellagitannins. Environmental and farming conditions can be exploited to obtain high quality blackberry fruits. Show more

Keywords: Blackberry, physicochemical properties, anthocyanins, ellagitannins, environmental conditions, farming practices

DOI: 10.3233/JBR-180353

Citation: Journal of Berry Research, vol. 9, no. 2, pp. 283-296, 2019

Authors: El-Mogy, Mohamed M. | Ludlow, Richard A. | Roberts, Callum | Müller, Carsten T. | Rogers, Hilary J.

Article Type: Research Article

Abstract: BACKGROUND: Strawberries are perishable fruits that decay quickly after harvest, but are valued for their distinctive taste and aroma. Melatonin is involved in plant resistance against stress, plant senescence and fruit ripening, and was shown to delay post-harvest spoilage of strawberries. OBJECTIVE: The effects of melatonin postharvest treatment on shelf-life and volatile organic compound profile were assessed in strawberry fruits cv “Luca”. METHODS: Strawberry fruit were treated with 100 μM melatonin and stored at 4°C for 12 days to assess whether melatonin treatment could delay spoilage without adversely affecting aroma. RESULTS: Melatonin treatment delayed …fruit deterioration by reducing weight loss and incidence of decay as well as maintaining total soluble solids, titratable acidity, anthocyanin, and taste. Melatonin treatment also significantly reduced CO2 production compared to control fruits. The relative abundance of the majority of volatile organic compounds (VOCs) was not affected, however abundance of two VOCs that are important components of strawberry aroma were affected by melatonin treatment. CONCLUSIONS: Post-harvest treatment of strawberries with 100 μM melatonin improved strawberry quality and conserved bioactive compounds after 12 d of storage. However, components of the aroma profile were altered in a way which may affect consumer perception of quality. Show more

Keywords: Ethyl hexanoate, fragaria×ananassa , melatonin, postharvest quality, volatile organic compounds

DOI: 10.3233/JBR-180361

Citation: Journal of Berry Research, vol. 9, no. 2, pp. 297-307, 2019

Authors: Canturk, Sevil | Kunter, Birhan | Buyukkartal, Hatice Nurhan

Article Type: Research Article

Abstract: BACKGROUND: Berry skin tissue is important in viticulture and enology due to the storage of phenolic compounds. Changes in the berry metabolism and environmental factors may lead to changes in skin structure and composition. OBJECTIVE: We aimed to determine the effects of kaolin-based particle film (PF) treatment on histological properties of anthocyanin accumulation in the berries of cv. Beauty Seedless and Tekirdag Seedless in two growing seasons. METHODS: PF treatment was applied 3 times beginning from the berry set. Histological structure of berries was observed with optical and transmission electron microscopy. Accumulation of anthocyanins in berry …skin cells was determined microscopically and spectrophotometrically. RESULTS: In Beauty Seedless, total anthocyanin content was 512.93–605.90 mgkg–1 in control (C) and it raised to 606.98–666.44 mgkg–1 in PF treated vines. Total anthocyanin content was determined as 72.50–81.43 mgkg–1 (C) and 79.55–84.72 mgkg–1 (PF) in Tekirdag Seedless. 3 cell types were observed regarding anthocyanin localization: cells with no anthocyanin (type 1), cells with vacuolar storage (type 2) and cells with granular storage (type 3). CONCLUSION: We concluded that the PF treatment could be used for increasing anthocyanin concentration with cultivar-specific skin color in Beauty Seedless and Tekirdag Seedless grown in the semi-arid regions. Show more

Keywords: Vitis vinifera L., anthocyanin concentration, anthocyanic vacuolar inclusions (AVI), berry skin, histochemistry

DOI: 10.3233/JBR-180323

Citation: Journal of Berry Research, vol. 9, no. 2, pp. 309-319, 2019

Authors: Jiang, Bo | Zheng, Shangli | An, Lijia | Guo, Jianli | Asakawa, Tetsuya

Article Type: Research Article

Abstract: BACKGROUND: Blueberry juice is a common drink for relieving fatigue, but its mechanisms of action are unclear. OBJECTIVE: This study aimed to validate changes in the dopaminergic system, especially changes in dopamine transporter (DAT) levels, in a fatigued state and assess whether such fatigue-related changes can be ameliorated by blueberry juice. METHODS: We investigated the expression of striatal DAT and tyrosine hydroxylase (TH) in mice subjected to a forced swimming fatigue task and subsequently administered blueberry juice (anthocyanin), caffeine, or Red Bull (caffeine + taurine) to assess DAT and TH changes in the nigrostriatal pathway related …with fatigue as well as their relief. RESULTS: Striatal DAT and TH expression was downregulated by fatigue and upregulated by treatment with blueberry juice, caffeine, and Red Bull. In addition, DAT and TH expression exhibited positive correlations with fatigue-related motor performance. CONCLUSIONS: We provided evidence of compensatory responses of striatal DAT and TH in the fatigued state, and treatments to relieve fatigue might upregulate the expression of both striatal DAT and TH. Moreover, small amounts of blueberry juice exerted similar effects as large amounts, suggesting that anthocyanin has relatively strong anti-fatigue effects. Show more

Keywords: Fatigue, anthocyanin, tyrosine hydroxylase, dopamine transporter, forced swimming test, blueberry juice

DOI: 10.3233/JBR-180350

Citation: Journal of Berry Research, vol. 9, no. 2, pp. 321-332, 2019

Authors: El-Mogy, Mohamed M. | Ali, Marwa R. | Darwish, Omaima S. | Rogers, Hilary J.

Article Type: Research Article

Abstract: BACKGROUND: Strawberry is one of the most highly consumed fruits worldwide. However, it is highly a perishable fruit postharvest. OBJECTIVE: To assess the effect of dipping strawberry fruits after harvest in plant growth regulators to maintain postharvest quality. METHODS: Treatments tested were: 2 and 4 mM salicylic acid (SA), 0.25 and 0.50 mM abscisic acid (ABA) and methyl jasmonate at 0.25 and 0.50 mM (MeJA). Bioactive compounds and fungal growth were assessed over 12 days of storage at 4°C. RESULTS: Both concentrations of SA and MeJA significantly suppressed weight loss, decay and respiration rate and 0.50 mM ABA …also reduced decay. Both concentrations of SA retarded color development, and total soluble solids content was enhanced by 0.50 mM ABA and MeJA treatments. The most effective treatments for preserving firmness were 0.25 mM MeJA and 4 mM SA. Reduction in loss of ascorbic acid and bioactive compounds during storage was achieved using the highest concentrations of SA, ABA, and MeJA. Fungal growth was suppressed by all treatments but the best treatment was MeJA at both concentrations. CONCLUSIONS: All three plant growth regulators reduce postharvest changes in strawberry but effects differ amongst the treatments. Show more

Keywords: Fragaria × ananassa, quality, postharvest storage, bioactive compounds

DOI: 10.3233/JBR-180349

Citation: Journal of Berry Research, vol. 9, no. 2, pp. 333-348, 2019

Authors: Čanadanović-Brunet, Jasna | Tumbas Šaponjac, Vesna | Stajčić, Slađana | Ćetković, Gordana | Čanadanović, Vladimir | Ćebović, Tatjana | Vulić, Jelena

Article Type: Research Article

Abstract: BACKGROUND: Considerable amounts of polyphenolic antioxidants remain in the press-cake during processing of berry fruits. OBJECTIVE: The aim of this study was to investigate polyphenolic profile, antiradical and hepatoprotective activities of wild bilberry (WBI) and blackberry cv. Čačanska bestrna (CBL) and cv. Thornfree (TBL) pomace extracts. METHODS: Polyphenolic profiles of berry pomace extracts were determined by HPLC. in vitro antiradical activities were confirmed with electron spin resonance (ESR) spectroscopy. In vivo antioxidant and hepatoprotective properties were evaluated by following activities of several enzymes and the levels of glutathione-GSH and thiobarbituric acid reactive substances. …RESULTS: Protocatechuic acid (3.36–35.18 mg g-1 ) and gallic acid (9.57–31.98 mg g-1 ) were the most dominant phenolic compounds in WBI, CBL and TBL. Strongest scavenging activity on O2 •- (EC50 O2  = 60.21 mmol TE g-1 ) and OH• (EC50 OH  = 3.00 mmol TE g-1 ) were found for WBI and CBL pomace extracts, respectively. The best result in neutralizing oxidative stress induced by CCl4 , was observed for 2 mL of berry pomace extracts kg-1 b.w. Simultaneous application of 0.1-2 mL berry pomace extracts kg-1 b.w. and CCl4 returned GSHPx, CAT and GSH levels to physiological levels. CONCLUSIONS: Bilberry and blackberry pomace are a good source of bioactive compounds with potential application in supplements and functional food. Show more

Keywords: Berries, HPLC, antioxidant enzymes, glutathione, thiobarbituric acid reactive substances

DOI: 10.3233/JBR-180362

Citation: Journal of Berry Research, vol. 9, no. 2, pp. 349-362, 2019