Cancer Biomarkers - Volume 22, issue 4

Authors: Zhong, Hua | Yang, Jun | Zhang, Bin | Wang, Xiaofang | Pei, Lihong | Zhang, Lei | Lin, Zhiqiang | Wang, Yanan | Wang, Chengbin

Article Type: Research Article

Abstract: Breast cancer is the most common malignancy in women which increases gradually all over the world. LncRNA GACAT3 has been found to be increased in gastric cancer and associated with tumor malignancy. However, whether GACAT3 plays a role in the regulation of breast cancer is not known. In the present study, we found that GACAT3 expression was increased in breast cancer tissues and cells compared with adjacent normal tissues and normal cells. High GACAT3 expression was correlated with the poor prognosis of breast cancer patients. GACAT3 and cyclin D2 (CCND2) contained a binding site of miR-497. miR-497 was decreased in …breast cancer tissues and cells compared with adjacent normal tissues and normal cells. Low miR-497 expression was correlated with the poor prognosis of breast cancer patients. In breast cancer tissues, the expression of miR-497 was negatively correlated with GACAT3. Downregulation of GACAT3 increased miR-497 expression. miR-497 mimic reduced the luciferase of GACAT3 and CCND2. Anti-miR-497 reversed the effects of GACAT3 downregulation. We also found that GACAT3 may act as a ceRNA for miR-497, enhancing the expression of CCND2. In conclusion, GACAT3 promotes breast cancer malignancy by sponging miR-497, leading to the enhancement of its endogenous target CCND2. These results suggest that GACAT3/miR-497/CCND2 is a potential therapeutic target and biomarker for breast cancer. Show more

Keywords: Breast cancer, CCND2, lncRNA GACAT3, malignancy, miR-497

DOI: 10.3233/CBM-181354

Citation: Cancer Biomarkers, vol. 22, no. 4, pp. 787-797, 2018

Authors: Huang, Yiqun | Zou, Yong | Lin, Luhui | Ma, Xudong | Chen, Hongpu

Article Type: Research Article

Abstract: BACKGROUND: MicroRNA-34a (miR-34a), as a tumor-suppressive miRNA, has been found to induce cell apoptosis in acute myeloid leukemia (AML). However, the diagnostic and prognostic significance of miR-34a in AML remains largely unknown. OBJECTIVE: We aimed to explore its associations with clinical characteristics and prognosis of AML patients. METHODS: This study detected serum miR-34a level in 117 diagnosed AML patients and 60 control subjects by using qRT-PCR, and results were compared to clinical features and patient outcome. Since cytogenetically-normal AML (CN-AML) has a good uniformity of cytogenetics and provides a perfect platform for detection of AML biomarkers, we …further analyzed miR-34a expression in 56 CN-AML subjects. RESULTS: We found that miR-34a was significantly downregulated in AML and CN-AML patients. MiR-34a underexpression was commonly observed in AML patients with intermediate/poor risk cytogenetic, and M5 subtype. ROC analysis demonstrated that serum miR-34a could well identify AML/CN-AML patients from healthy individuals. More importantly, miR-34a expression was found negatively correlated with aggressive clinical variable, and served as an independent prognostic indicator. In addition, AML/CN-AML patients with low miR-34a expression displayed shorter overall and recurrence free survival. CONCLUSIONS: Altogether, miR-34a might have an application as a diagnostic and prognostic indicator for AML patients. Show more

Keywords: miR-34a, biomarker, acute myeloid leukemia, diagnosis, prognosis

DOI: 10.3233/CBM-181381

Citation: Cancer Biomarkers, vol. 22, no. 4, pp. 799-805, 2018

Authors: Jia, Tao | Zhang, Run | Zhu, Hua-Yuan | Liang, Jin-Hua | Wang, Li | Wu, Wei | Cao, Lei | Li, Jian-Yong | Xu, Wei

Article Type: Research Article

Abstract: BACKGROUND: Peripheral blood absolute monocyte count (AMC) and lymphocyte to monocyte ratio (LMR) have strong prognostic value in various forms of lymphomas. It was found that higher AMC and lower LMR were associated with poor prognosis in B cell lymphoma. However, their prognostic significance in systemic anaplastic large cell lymphoma (sALCL) remained to be determined. OBJECTIVE: This study was aimed at investigating the prognostic significance of AMC and LMR in sALCL. METHODS: A total of 29 newly diagnosed patients with sALCL were retrospectively included in study, prognostic significance of AMC, LMR, performance status …(PS), international prognostic index (IPI), prognostic index for T-cell lymphomas (PIT) and other indicators were analyzed. RESULTS: Univariate analysis showed high AMC, LMR < 2.5, PS ⩾ 2, extranodal involvement, no response to treatment and B symptoms predicted inferior PFS; LMR < 2.5, no response to treatment, elevated LDH, IPI ⩾ 3, PIT ⩾ 2 and PS ⩾ 2 predicted inferior OS. High AMC, PS ⩾ 2 were independent prognostic factors for PFS; PS ⩾ 2, no response to treatment and elevated LDH were independent prognostic factors for OS. CONCLUSIONS: AMC was an independent prognostic factor for PFS in sALCL, and LMR < 2.5 also indicated poor prognosis. Show more

Keywords: Anaplastic large cell lymphoma, absolute monocyte count, absolute lymphocyte count, lymphocyte to monocyte ratio, prognosis

DOI: 10.3233/CBM-181505

Citation: Cancer Biomarkers, vol. 22, no. 4, pp. 807-813, 2018