Cancer Biomarkers - Volume 16, issue 3

Authors: Li, Zhen | Shu, Jinian | Zhang, Peng | Sun, Wanqi | Yang, Bo | Zhang, Haixu

Article Type: Research Article

Abstract: BACKGROUND: Identifying endogenous volatile organic compounds (VOCs) as markers for different cancers currently requires time-consuming procedures and specialized operators. OBJECTIVE: The objective of this study was to develop a rapid and simple method for measuring VOCs at trace levels. METHODS: A simple vacuum ultraviolet photoionization mass spectrometer (VUV-PIMS) was used to detect trace levels of dimethyl trisulfide (DMTS), dimethyl sulfide (DMS), and 2-butanone, which correspond to volatile biomarker candidates present in the exhaled breath of patients with breast, liver, and lung cancers, respectively. The practicality of measuring endogenous VOCs using VUV-PIMS was confirmed …by detecting them in cultured cell lines. RESULTS: The abovementioned VOCs were detected with high sensitivity by VUV-PIMS. The limits of detection (LODs) for DMTS, DMS, and 2-butanone were 3.1, 3.9, and 23.2 pptv, respectively, under ambient conditions, which surpass the sensitivity of nearly all other MS-based techniques. Moreover, relatively high concentrations of 2-butanone and DMS were observed in VOCs emitted from the A549 lung cancer cell line and the HepG2 liver cancer cell line, respectively. CONCLUSIONS: Our results show that VUV-PIMS may serve as a reliable method for real-time measurement of endogenous volatile cancer biomarkers. Show more

Keywords: VUV-PIMS, cancer, endogenous VOCs, cell measurement

DOI: 10.3233/CBM-160587

Citation: Cancer Biomarkers, vol. 16, no. 3, pp. 477-487, 2016

Authors: Kanmaz, Zehra Dilek | Aras, Gülfidan | Tuncay, Esin | Bahadır, Ayşe | Kocatürk, Celalettin | Yaşar, Zehra Asuk | Öz, Büge | Özkurt, Canan Ünlü | Gündoğan, Cihan | Çermik, Tevfik Fikret

Article Type: Research Article

Abstract: AIM: The aim of this study is to evaluate the diagnostic value of PET-CT scan for the prediction of EGFR mutation status and the contribution of TTF-1 expression to PET-CT scan. METHODS: We retrospectively studied 218 cases with a diagnosis of pulmonary adenocarcinoma between 2012-2014 which underwent EGFR analysis, TTF-1 and PET-CT before treatment. RESULTS: The EGFR mutation was present in 28.9% (n= 63) of cases. TTF-1 positivity was 66.9% (n= 105). Standardized uptake value (SUV max) was 16.7 ± 6.8 in EGFR mutant type, 13.8 ± 7.6 in cases having no …EGFR mutations. According to our evaluations, high SUVmax is positively correlated with EGFR mutation status. TTF-1 expression in multivariate analysis strengthens the accuracy of detecting an EGFR mutation. CONCLUSION: PET-CT FDG uptake may, together with TTF-1 expression, help diagnosis in lung adenocarcinoma cases when evaluating for EGFR mutation status. Show more

Keywords: Lung cancer, EGFR, TTF-1

DOI: 10.3233/CBM-160588

Citation: Cancer Biomarkers, vol. 16, no. 3, pp. 489-498, 2016

Authors: Kazemzadeh, Mina | Safaralizadeh, Reza | Feizi, Mohammad Ali Hosseinpour | Ravanbakhsh, Reyhaneh | Somi, Mohammad Hossein | Hashemzadeh, Shahryar

Article Type: Research Article

Abstract: Colorectal cancer (CRC) is one of the most common cancers in the world; therefore, extensive research is needed to find new molecular therapeutic targets and biomarkers. LncRNA (long non-coding RNA), a new class of non-coding RNAs, has a crucial role in the onset and progression of various cancersincluding colorectal cancer. Research on lncRNA is still at initial stages and underlying molecular mechanisms of the vast majority of lncRNA have remained unclear. LOC100287225 is one of these novel lncRNAs (long intergenic non-coding RNA) located in the long arm of the chromosome 18. The purpose of this study was to determine the …expression of LOC100287225 in colorectal tissue, and its misregulation in CRC patients. Quantitativereal-time-PCR (qRT-PCR) was used to investigate the LOC100287225 expression in pairs of tumorous and adjacent tumor-free tissues of 39 colorectal cancer patients. Also, the relationship between the clinicopathology and expression of LOC100287225 was determined. QRT-PCR results revealed that not only is LOC100287225 expressed in the intestinal tissue, but has also been misregulated during tumorigenesis. Moreover, LOC100287225 RNA relative expression levels were significantly lower in tumor tissues compared with adjacent tumor-free tissues (P< 0.001). RNA expression level of LOC100287225 did not show significant correlation with clinical characteristics. In conclusion, our study demonstrated that LOC100287225 misregulation couldbe a potential target for gene therapy in colorectal cancer. Show more

Keywords: CRC, lincRNA, LOC100287225, qRT-PCR

DOI: 10.3233/CBM-160589

Citation: Cancer Biomarkers, vol. 16, no. 3, pp. 499-505, 2016