Affiliations: [a] Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran | [b] Liver and Gastroenterology Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran | [c] Liver and Gastrointestinal Disease Research Center and Department of General and Thoracic Surgery, Tabriz University of Medical Sciences, Tabriz, Iran
Correspondence:
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Corresponding author: Reza Safaralizadeh, Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran. Tel.: +98 41 33392694; Fax: +98 41 33300037; E-mail:[email protected]
Abstract: Colorectal cancer (CRC) is one of the most common cancers in the world;
therefore, extensive research is needed to find new molecular therapeutic
targets and biomarkers. LncRNA (long non-coding RNA), a new class of
non-coding RNAs, has a crucial role in the onset and progression of various
cancersincluding colorectal cancer. Research on lncRNA is still at initial
stages and underlying molecular mechanisms of the vast majority of lncRNA
have remained unclear. LOC100287225 is one of these novel lncRNAs (long
intergenic non-coding RNA) located in the long arm of the chromosome 18. The
purpose of this study was to determine the expression of LOC100287225 in
colorectal tissue, and its misregulation in CRC
patients. Quantitativereal-time-PCR (qRT-PCR) was used to investigate the
LOC100287225 expression in pairs of tumorous and adjacent tumor-free tissues
of 39 colorectal cancer patients. Also, the relationship between the
clinicopathology and expression of LOC100287225 was determined. QRT-PCR
results revealed that not only is LOC100287225 expressed in the intestinal
tissue, but has also been misregulated during tumorigenesis. Moreover,
LOC100287225 RNA relative expression levels were significantly lower in
tumor tissues compared with adjacent tumor-free tissues (P< 0.001). RNA
expression level of LOC100287225 did not show significant correlation with
clinical characteristics. In conclusion, our study demonstrated that
LOC100287225 misregulation couldbe a potential target for gene therapy in
colorectal cancer.
