This study was conducted in a nursery for eucalyptus seedling production in a forest Company, located in the southern state of Bahia, Brazil. It aimed to evaluate the ergonomic conditions of the preparation of cuttings and mini-cuttings with scissors for Eucalyptus seedling production to increase well-being, satisfaction and safety and minimizing the occurrence of occupational diseases, also improving the process efficiency and final product quality. Environmental conditions, levels of noise, postures, RSI risk and physical workload were assessed. The environmental conditions complied with the recommended norms, except at certain times of day, when workers should make compensation breaks. The workers that collect minicuttings presented postures classified as normal by OWAS. On the other hand, the posture of workers dealing with cutting preparation requires corrections in a short run. The workers were subjected to moderate RSI risk due to the high degree of repeatability found. The physical workload was classified as mild, with no need for ergonomic interventions.