Affiliations: Laboratory of Physical Chemistry, Department of Chemistry, Faculty of Science, University of Guilan, Rasht, Iran
Note: [] Corresponding author: Hamid Dezhampanah, Laboratory of Physical Chemistry, Department of Chemistry, Faculty of Science, University of Guilan, P.O. Box 1914, Rasht 0098, Iran. Tel.: +98 131 3243630 5; Fax: +98 131 3233262; E-mails: [email protected]; [email protected].
Abstract: The interaction between anionic form of copper (II) N,N′,N″,N‴-tetrasulfonated phthalocyanine Cu (tspc) and to calf thymus deoxyribonucleic acid (ct-DNA) is investigated by measuring UV-vis absorption and fluorescence spectroscopy in phosphate buffer. The binding constant and stoichiometry were determined by analysis of optical absorption spectra of phthalocyanine at various ct-DNA concentrations using SQUAD software. The static mode of fluorescence quenching of phthalocyanine by calf thymus deoxyribonucleic acid indicates the formation of a ground-state complex. The formation of ground-state complex is a spontaneous molecular interaction procedure in which outside groove binding through the formation of an axial bond between the base pairs of nucleotide and Cu in the central core of phthalocyanine.
Keywords: DNA, phthalocyanine, thermodynamic of binding, optical absorption, SQUAD
DOI: 10.3233/SPE-2012-0564
Journal: Spectroscopy, vol. 26, no. 6, pp. 357-365, 2011
