Affiliations: Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou, Fujian, China | College of Physics and Information, Fuzhou University, Fuzhou, Fujian, China | Fujian Provincial Tumor Hospital, Fuzhou, Fujian, China | Cancer Imaging Department, British Columbia Cancer Research Centre, Vancouver, BC, Canada
Note: [] Yuhuang Ye and Yang Chen are co-first authors; they contributed equally to the work.
Note: [] Corresponding author: Prof. Rong Chen, Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou 350007, China. Tel.: +86 591 8348 9919; Fax: +86 591 8346 5373; E-mail: [email protected].
Abstract: Raman microspectroscopy can provide molecular-level information about the biochemical composition and structure of cells and tissues with excellent spatial resolution. In this study, Raman spectroscopy of individual cells from nasopharyngeal carcinoma cell lines C666-1, CNE2 and nasopharyngeal normal cell line NP69 are investigated for their differences. The spectral intensity ratio at 1449 and 1657 cm−1 with a decision line of I1449/I1657=1.10 can very easily separate the tumor and normal cell lines into two groups. Principal component analysis (PCA) and linear discriminant analysis (LDA) are also used to classify different cell lines and achieved a specificity and sensitivity of 100 and 90%, respectively. The results support the potential utility of Raman spectroscopy for nasopharyngeal diagnosis.
Keywords: Confocal Raman microspectroscopy, nasopharyngeal carcinoma cells, principal component analysis
DOI: 10.3233/SPE-2011-0511
Journal: Spectroscopy, vol. 25, no. 5, pp. 217-224, 2011
