Affiliations: Clinical Materials Unit, Materials and Surface Science Institute, University of Limerick, Limerick, Ireland | Interface Analysis Centre, University of Bristol, Bristol, UK | Department of Health, Sport and Exercise Science, Waterford Institute of Technology, Waterford, Ireland | Department of Physical Education and Sports Science, University of Limerick, Limerick, Ireland | Inamori School of Engineering, Alfred University, Alfred, NY, USA
Note: [] Corresponding author: Dr. Niamh M. Cummins, Clinical Materials Unit, Materials and Surface Science Institute, University of Limerick, National Technological Park, Limerick, Ireland. Tel.: +353 61 234170; Fax: +353 61 338172; E-mail: [email protected].
Abstract: Raman data from human fingernails has been analysed to determine if a relationship exists between spectral features and bone quality. Previous work demonstrated a relationship between the manually determined intensity of the disulphide peak (Bone Quality Test; BQT) and osteoporotic fracture. A computer program is now described that automates the BQT determination for large sample-sets. In this study the ability of the automated BQT to discriminate between fractures and controls was compared to that of bone mineral density (BMD) and biomarkers of bone remodelling. Females aged 18–67 years participated in the study (n=159). Fingernails were analysed using Raman spectroscopy. Lumbar and Femoral BMD was measured by dual energy X-ray absorptiometry (DXA). Fasting venepuncture samples were analysed for osteocalcin and CTx (collagen C-telopeptides) by electrochemiluminescence. No correlation was found between the BQT and BMD or the biomarkers. The BQT and BMD were found to be equally accurate in identifying subjects with a history of fracture (p<0.01) and both outperformed osteocalcin and CTx. It appears that keratin and collagen are related structural proteins that require disulphide bonding for stability. Therefore, these preliminary results suggest that Raman spectroscopy of keratin may have potential as a diagnostic tool for screening bone quality in large populations.
Keywords: Raman spectroscopy, osteoporosis, fracture, fingernails, bone density, biomarker
DOI: 10.3233/SPE-2010-0471
Journal: Spectroscopy, vol. 24, no. 5, pp. 517-524, 2010
