Abnormal shifts in Raman spectra of deuterated cytidine and 6-azacytidine

Issue title: From Molecule to Tissue: XIII European Conference on the Spectroscopy of Biological Molecules, Palermo, Italy, August 28–September 2, 2009, Part 2 of 2

Article type: Research Article

Authors: Garasevych, S. | Iakhnenko, M. | Slobodyanyuk, O. | Vaskivskyi, I.

Affiliations: Department of Physics, Taras Shevchenko National University of Kyiv, Kyiv, Ukraine

Note: [] Corresponding author: Marianna Iakhnenko, Department of Physics, Taras Shevchenko National University of Kyiv, 64 Volodymyrs'ka St., 01033 Kyiv, Ukraine. Tel.: +38 0506 353696; Fax: +38 0442 854678; E-mail: [email protected].

Abstract: It was revealed that some peaks in Raman spectra of cytidine, 6-azacytidine (6-azaC), and cytosine dissolved in D2O are shifted to high frequencies in respect to their positions in spectra of H2O solution. Such “blue shifts” occur due to deuteration of the nucleoside molecule itself but not due to effect of deuterated solvent. This conclusion is deduced from observation of blue (abnormal) shift in Raman spectra of cytidine and 6-azaC microcrystals recrystallized from D2O solution. Both normal and abnormal shifts close to the experimentally observed were obtained in the calculated spectra of 6-azaC and cytidine. We assume that abnormal shifts may be caused by substitution of intramolecular H-bonds with D-bonds.

Keywords: Raman spectra, cytidine, 6-azacytidine, blue shift, H-bonds, deuteration

DOI: 10.3233/SPE-2010-0461

Journal: Spectroscopy, vol. 24, no. 3-4, pp. 191-195, 2010