Affiliations: Department of Engineering Technology, Jiangsu Institute of Economic and Trade Technology, Nanjing, P. R. China | Research and Development Centre of Food Safety Engineering Technique of Jiangsu, Nanjing, P. R. China
Note: [] Corresponding author: Xiao-Hua Li, Department of Engineering Technology, Jiangsu Institute of Economic and Trade Technology and Research and Development Centre of Food Safety Engineering Technique of Jiangsu, Nanjing 210007, Jiangsu Province, P. R. China. Tel.: +86 25 86569303; E-mail: [email protected].
Abstract: The interaction between bovine serum albumin (BSA) and prulifloxacin was investigated by ultraviolet spectrophotometer (UV) and fluorescence spectroscopy in this paper. Two-dimensional (2D) correlation spectroscopy was applied to the analysis of fluorescence spectra. The results of spectroscopic measurements suggested that prulifloxacin (PL) have a strong ability to quench the intrinsic fluorescence of bovine serum albumin through static quenching procedure. Thermodynamic parameter enthalpy changes (ΔH) and entropy changes (ΔS) were calculated. Owing to the spectral resolution enhancement in 2D correlation spectroscopy, the structure change of prulifloxacin can be observed.
