Affiliations: School of Life Sciences, University of Science and Technology of China, Hefei 230026, China | School of Life Sciences, Graduate University of CAS, Beijing 100049, China | School of Life Sciences, Jiling University, Changchun 130000, China | Institute of High Energy Physics, The Chinese Academy of Sciences, Beijing 100049, China
Note: [] Corresponding author: Dr. Jianhong Yang, School of Life Sciences, Graduate University of Chinese Academy of Sciences (CAS), 19(A) Yuquan Road, Beijing 100049, P. R. China. Tel.: +86 010 88256349; Fax: +86 010 88256080; E-mail: [email protected].
Abstract: To evaluate the anti-osteoporotic effect of antler blood, the relationship between the change of element content and the bone mineral density (BMD) increase in femur was investigated. Female Wistar rats were randomly divided into three groups: sham-operated group (SHAM, n=5), ovariectomized group (OVX, n=5) and ovariectomized group with antler blood treatment (n=5). The femoral BMD was analyzed by dual-energy X-ray absorptiometry (DXA) and the element relative content was determined by synchrotron radiation X-ray fluorescence (SRXRF) microprobe. The results showed that the femoral BMD in ovariectomized rats was significantly lower than that of sham-operated rats (p<0.05) but reversed by antler blood treatment (p<0.05). A further study demonstrated that the relative contents of phosphorus (P), calcium (Ca), zinc (Zn) and strontium (Sr) were obviously lower in ovariectomized rats compared to sham-operated rats but only the relative contents of P, Ca and Zn were normalized by antler blood treatment (p<0.05). Our experiments revealed that loss of element Ca, P, Zn and Sr was closely related to the BMD reduction in ovariectomized rats and the anti-osteoporotic effect of antler blood was mediated by increasing the contents of P, Ca and Zn.
Keywords: Element analysis, BMD, osteoporosis, femur, SRXRF
DOI: 10.3233/SPE-2009-0372
Journal: Spectroscopy, vol. 23, no. 1, pp. 1-9, 2009
