FTIR-ATR biosensor based on self-assembled phospholipids surface: Haemophilia factor VIII diagnosis
Issue title: From Molecule to Tissue: XII European Conference on the Spectroscopy of Biological Molecules, Bobigny, France, 1–6 September 2007, Part 2 of 2
Affiliations: Laboratory for the Structure and Function of Biological Membranes, Center for Structural Biology and Bioinformatics, Free University of Brussels, Campus Plaine CP206/2, 1050 Brussels, Belgium
Note: [] Corresponding author (current address): Département des sciences de la matière, Faculté Polydisciplinaire, Université Cadi Ayyad, BP-4162, 46000, Safi, Morocco. Tel.: +212 64310123; Fax: +212 24669516; E-mail: [email protected].
Abstract: We report a new generic device suitable for the investigation of biological interactions by means of Fourier Transform Infrared (FTIR) spectroscopy. The research is focused on multi reflected evanescent optical radiation through a chemically modified surface of the attenuated total reflection (ATR) element (silicon or germanium). Using a wet chemistry approach, the original method is based on grafting of a bifunctional binding molecule (N-hydroxysuccinimidyl ester forms) at the surface of the Si or the Ge crystals. The functionalized surface permits then the foundation of different types of self-assembled phospholipid membranes. The obtained sensors allow the detection, in infrared spectral domain, of any perceptible molecular interaction or structural changes. The key experimental result concerns the coagulation factor VIII (FVIII). The principle of the diagnostic is related to the ability of FVIII molecules to bind specifically to phosphatidylserine (PS) membrane.
