Synchrotron Radiation Circular Dichroism (SRCD) spectroscopy: New beamlines and new applications in biology

Article type: Research Article

Authors: Miles, Andrew J. | Hoffmann, Søren Vrønning | Tao, Ye | Janes, Robert W.^; | Wallace, B.A.^;

Affiliations: Department of Crystallography, Birkbeck College, University of London, London WC1E 7HX, UK | Institute for Storage Ring facilities (ISA), Aarhus University, Aarhus, Denmark | Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, China | School of Biological and Chemical Sciences, Queen Mary, University of London, London E1 4NS, UK

Note: [] Corresponding author: R.W. Janes, Tel.: +44 207 882 3038; Fax: +44 208 983 0973; E-mail: [email protected].

Note: [] Corresponding author: B.A. Wallace, Tel.: +44 207 631 6800; Fax: +44 207 631 6803; E-mail: [email protected]. bbk.ac.uk.

Abstract: New advances in instrumentation, demonstration of proof-of-principle studies, and development of new tools and methods for data analysis and interpretation have enabled the technique of Synchrotron Radiation Circular Dichroism (SRCD) spectroscopy to become a useful tool for structural and functional biology. This paper discusses the characterisation of two new SRCD beamlines, CD1 at the Institute for Storage Rings (ISA), Denmark and 4B8 at the Beijing Synchrotron Radiation Facility (BSRF), China, and new applications of the method for examining biological systems.

Keywords: Synchrotron Radiation Circular Dichroism spectroscopy (SRCD), vacuum ultraviolet (VUV), structural biology, protein secondary structure, calibration

Journal: Spectroscopy, vol. 21, no. 5-6, pp. 245-255, 2007