Affinity studies of hypocrellin B and mono-cysteine substituted hypocrellin B with CT-DNA using spectroscopic methods

Article type: Research Article

Authors: Wei, Shaohua^{; ;} | Zhou, Jiahong | Feng, Yuying | Huang, Deyin | Wang, Xuesong^; | Zhang, Baowen^; | Shen, Jian

Affiliations: School of Chemistry and Chemical Technology, Shanghai JiaoTong University, Shanghai 200240, PR China | Jiangsu Engineering Research Center for Bio-medical Function Material, Nanjing Normal University, Nanjing 210097, PR China | Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100101, PR China

Note: [] Corresponding authors. Tel.: +86 25 8359 8253; Fax: +86 25 8359 8250; E-mail: [email protected].

Note: [] Corresponding authors. Tel.: +86 25 8359 8253; Fax: +86 25 8359 8250; E-mail: [email protected].

Note: [] Corresponding authors. Tel.: +86 25 8359 8253; Fax: +86 25 8359 8250; E-mail: [email protected].

Abstract: The interaction of anticancer drug hypocrellin B (HB) and mono-cysteine substituted hypocrellin B (MCHB) with calf thymus deoxyribonucleic acid (CT-DNA) has been investigated using spectral methods. The results of UV–visible spectra showed that the HB and MCHB can intercalate into the base-stacking domain of the CT-DNA double helix. Further studies based on fluorescence spectroscopy and circular dichroism (CD) spectroscopy also supported the intercalation mechanism.

Keywords: Hypocrellin B, MCHB, CT-DNA, intercalation mechanism, circular dichroism spectroscopy, fluorescence spectroscopy, anticancer drugs

Journal: Spectroscopy, vol. 19, no. 5-6, pp. 259-266, 2005