Probing the role of metal ions on reversible peptide–protein interactions by NMR

Issue title: Second International Conference on Biomedical Spectroscopy: From the Bench to the Clinic, London, UK, 5–8 July, 2003

Article type: Research Article

Authors: D'Amelio, Nicola | Gaggelli, Elena | Gaggelli, Nicola | Mancini, Francesca | Molteni, Elena | Valensin, Daniela | Valensin, Gianni

Affiliations: Department of Chemistry and the NMR Center, University of Siena, Via A.Moro, Siena 53100, Italy

Note: [] Corresponding author. Tel.: +39 577 234243; Fax: +39 577 234254; E‐mail: [email protected].

Abstract: This work provides evidence that paramagnetic lanthanide ions constitute ideal probes suitable for investigations of metal effects upon peptide–receptor interactions with the use of NMR methods. Cerium(III) is herein used for assessing metal effects upon the interaction between angiotensin II and a fragment from the AT1A receptor. Angiotensin II forms a complex with cerium(III) in water while the fCT300–320 receptor fragment is poorly affected by cerium(III). However, the addition of the fragment displaces cerium(III) from the complex, thus directly demonstrating the higher affinity of angiotensin II for the receptor and probing the peptide residues involved in receptor binding.

Keywords: NMR, [TeX:] ${}^{1}$H, angiotensin II, AT[TeX:] $_{1A}$ receptor, lanthanides, cerium(III)

Journal: Spectroscopy, vol. 18, no. 2, pp. 251-256, 2004