Using cryoprobes to decrease acquisition times of triple‐resonance experiments used for protein resonance assignments

Issue title: First International Conference on Biomedical Spectroscopy: From molecules to men, Cardiff, UK, 7–10 July 2002, Part II

Article type: Research Article

Authors: Goger, Michael J.^; | McDonnell, James M.^; | Cowburn, David

Affiliations: New York Structural Biology Center, 89 Convent Avenue, The Park Building at 133rd Street, New York, NY 10031, USA | Laboratory of Molecular Biophysics, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK

Note: [] Corresponding author. Tel.: +1 212 939 0660; Fax: +44 1865 275182; E‐mail: [email protected].

Abstract: In most structural biology NMR laboratories, instrument time is a limiting factor in the number of structural projects a laboratory is able to support. In the post‐genomic era we can expect the number of structural targets to markedly increase. Here we address to what degree recently introduced cryoprobes, which are 3–4 times as sensitive as conventional probes, can alleviate this problem. To evaluate this approach, a set of triple‐resonance experiments for protein assignments were acquired with a cryoprobe. We show that, with the cryoprobe, high quality triple‐resonance data can be obtained within as 4 hours/experiment. These results show that a full set of data for protein assignments can now be practically collected in 1–2 days.

Keywords: NMR spectroscopy, cryoprobes, triple‐resonance, proteins, high‐throughput, reduced phase cycle

Journal: Spectroscopy, vol. 17, no. 2-3, pp. 161-167, 2003