Affiliations: First Department of Anatomy, Semmelweis University Medical School, Budapest (Hungary) | Central Research Division, Postgraduate Medical University, Budapest (Hungary) | I.P.Pavlov Department of Physiology, Institute of Experimental Medicine, St. Petersburg (Russia)
Note: [] Correspondence: V.V. Senatorov, Neuroscience Research, Loeb Research Institute, Ottawa Civic Hospital, 1053 Carling Avenue, Ottawa, ON, KIY 4E9 Canada.
Note: [] Present address: Brain Research Laboratory, Institute of Animal Behavior, Rutgers – The State University of New Jersey, 101 Warren Street, Newark, NJ 07102, USA.
Abstract: Fetal cerebral cortical tissue was transplanted into an aspirated lesion cavity made in the sensorimotor cortex of adult rats. Ten weeks after grafting, outgrowing fibers from the graft were visualized by an anterograde tracing technique using Phaseolus vulgaris leucoagglutinin (PHA-L). It was demonstrated that the efferent fibers grew into the neighboring host cortical tissue, the corpus callosum and in some cases approached caudate/putamen. Characteristic axon arborization with abundant boutons were found in the host cortical tissue, but only in close vicinity to the grafts. It is concluded that the PHA-L anterograde tracing technique can be a useful tool to assess the degree of anatomical integration of the transplants into the host tissue.
