Constitutively reduced sensory capacity promotes better recovery after spinal cord-injury (SCI) in blind rats of the dystrophic RCS strain

Article type: Research Article

Authors: Rink, Svenja^{a; 1} | Bendella, Habib^{b; 1} | Alsolivany, Kurdin^{c; 1} | Meyer, Carolin^{d; 1} | Woehler, Aliona^e | Jansen, Ramona^c | Isik, Zeynep^c | Stein, Gregor^f | Wennmachers, Sina^c | Nakamura, Makoto^b | Angelov, Doychin N.^{c; *}

Affiliations: [a] Department of Prosthetic Dentistry, School of Dental and Oral Medicine, University of Cologne, Germany | [b] Department of Neurosurgery, University of Witten/Herdecke, Cologne Merheim Medical Center (CMMC), Cologne, Germany | [c] Department of Anatomy I, University of Cologne, Germany | [d] Department of Orthopedics and Traumatology, University of Cologne, Germany | [e] Bundeswehrzentralkrankenhaus, Koblenz, Germany | [f] Department of Orthopaedics and Trauma Surgery, Helios Klinikum Siegburg, Germany

Correspondence: [*] Corresponding author: Prof. Dr. Doychin N. Angelov, M.D., Ph.D., Institut für Anatomie der Universität zu Köln, Joseph-Stelzmann-Strasse 20, D-50931 Köln, Germany. Tel.: +49 221 478 5654; Fax: +49 221 478 87893; E-mail: [email protected].

Note: [1] These authors contributed equally and share first authorship.

Abstract: Background:We compared functional, electrophysiological and morphological parameters after SCI in two groups of rats Sprague Dawley (SD) rats with normal vision and blind rats from a SD-substrain “Royal College of Surgeons” (SD/RCS) who lose their photoreceptor cells after birth due to a genetic defect in the retinal pigment epithelium. For these animals skin-, intramuscular-, and tendon receptors are major available means to resolve spatial information. Objective:The purpose of this study was to check whether increased sensitivity in SD/RCS rats would promote an improved recovery after SCI. Methods:All rats were subjected to severe compression of the spinal cord at vertebra Th8, spinal cord segment Th10. Recovery of locomotion was analyzed at 1, 3, 6, 9, and 12 weeks after SCI using video recordings of beam walking and inclined ladder climbing. Five functional parameters were studied: foot-stepping angle (FSA), rump-height index (RHI) estimating paw placement and body weight support, respectively, number of correct ladder steps (CLS) assessing skilled hindlimb movements, the BBB-locomotor score and an established urinary bladder score (BS). Sensitivity tests were followed by electrophysiological measurement of M- and H-wave amplitudes from contractions of the plantar musculature after stimulation of the tibial nerve. The closing morphological measurements included lesion volume and expression of astro- and microglia below the lesion. Results:Numerical assessments of BBB, FSA, BS, lesion volume and GFAP-expression revealed no significant differences between both strains. However, compared to SD-rats, the blind SD/RCS animals significantly improved RHI and CLS by 6 – 12 weeks after SCI. To our surprise the withdrawal latencies in the blind SD/RCS rats were longer and the amplitudes of M- and H-waves lower. The expression of IBA1-immunoreactivity in the lumbar enlargement was lower than in the SD-animals. Conclusion:The longer withdrawal latencies suggest a decreased sensitivity in the blind SD/RCS rats, which promotes better recovery after SCI. In this way our results provide indirect support to earlier work showing, that hypersensitivity and chronic pain after contusive SCI impair the recovery of locomotor function.

Keywords: Compression spinal cord injury, blind SD/RCS rats, recovery of motor performance, functional parameters, electrophysiological measurements

DOI: 10.3233/RNN-170789

Journal: Restorative Neurology and Neuroscience, vol. 36, no. 3, pp. 397-416, 2018