Interleukin-1β and tumor necrosis factor-α co-operatively enhance a novel trophic activity of astrocytes for pontine cholinergic neurons in vitro

Article type: Research Article

Authors: Kamiguchi, Hiroyuki | Yoshida, Kazunari | Inaba, Makoto | Sasaki, Hikaru | Otani, Mitsuhiro | Toya, Shigeo

Affiliations: Department of Neurosurgery, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160, Japan

Note: [] Corresponding author. Present address: Department of Neurosciences, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA, Tel: + 1 216 368 2575; fax: + 1 216 368 4650.

Abstract: Insulin and insulin-like growth factors (IGFs) are the only known trophic factors for pontine cholinergic neurons. The present study revealed that astrocyte-extract pretreated with IL-1β and TNF-α significantly enhanced choline acetyltransferase (ChAT) activity of the pontine neurons in the presence of a supramaximal dose of insulin, while various trophic factors including IGFs failed to increase the ChAT activity under the same culture conditions, suggesting that IL-1β and TNF-α co-operatively enhanced the expression of a novel trophic factor for pontine cholinergic neurons in astrocytes.

Keywords: Astrocyte, effect, InterleukiNeurotrophicn-1β, Tumor necrosis factor-α, Pontine cholinergic neuron, Choline acetyltransferase

DOI: 10.3233/RNN-1996-10106

Journal: Restorative Neurology and Neuroscience, vol. 10, no. 1, pp. 43-47, 1996