Immunohistochemistry in pathology: A review

Article type: Research Article

Authors: Kohale, Mangesh G.^a | Dhobale, Anupama V.^{b; *} | Bankar, Nandkishor J.^c | Noman, Obaid^a | Hatgaonkar, Kajal^d | Mishra, Vaishnavi^e

Affiliations: [a] Department of Pathology, Datta Meghe Medical College, Nagpur, Datta Meghe Institute of Higher Education and Research (DU), Sawangi (Meghe), Wardha, India | [b] Department of Obstetrics and Gynaecology, Datta Meghe Medical College, Nagpur, Datta Meghe Institute of Higher Education and Research (DU), Sawangi (Meghe), Wardha, India | [c] Department of Microbiology, Jawaharlal Nehru Medical College, Wardha, Datta Meghe Institute of Higher Education and Research (DU), Sawangi (Meghe), Wardha, India | [d] Department of Pathology, Datta Meghe Medical College, Nagpur, Datta Meghe Institute of Higher Education and Research (DU), Wardha, Maharashtra, India | [e] Department of Microbiology, Jawaharlal Nehru Medical College Sawangi (Meghe) Wardha, Datta Meghe Institute of Higher Education and Research (DU), Wardha, Maharashtra, India

Correspondence: [*] Corresponding author: Anupama Dhobale, Assistant Professor, Department of Obstetrics and Gynaecology, Datta Meghe Medical College, Nagpur, India. Datta Meghe Institute of Higher Education and Research (DU), Sawangi (Meghe), Wardha, India. E-mail: [email protected].

Abstract: Immunohistochemistry (IHC) is an invaluable technique in pathology that allows for the localization and characterization of specific antigens within tissues using specific antibodies. Over the years, IHC has revolutionized the field of diagnostic pathology, providing critical information about the molecular and cellular composition of tissues. This comprehensive review article aims to provide an overview of the principles, methodologies, applications, and challenges of IHC in pathology. Immunohistochemistry (IHC) is a vital technique in pathology that allows for the detection and visualization of specific antigens within tissue samples. This article provides an overview of the principles, methodology, and applications of IHC in pathology. The principles of immunoreactivity, antibody selection, and detection methods are discussed, highlighting their importance in achieving accurate and reliable results. The methodology of IHC, including tissue preparation, antigen retrieval, and visualization techniques, is outlined to guide researchers and pathologists in implementing the technique effectively. The article also explores the role of antibodies in IHC, emphasizing the differences between monoclonal and polyclonal antibodies, as well as the significance of antibody validation and quality control. The applications of IHC in pathology are described, ranging from tumor classification and prognostic assessments to the detection of infectious agents and the characterization of autoimmune diseases. Furthermore, the article examines advancements and challenges in IHC, such as multiplex staining, digital pathology, quality control, and interpretation. Finally, emerging techniques, including the integration of next-generation sequencing and artificial intelligence, are discussed as future perspectives for IHC. Overall, this comprehensive overview demonstrates the indispensable role of IHC in pathology and its continuous evolution to meet the ever-expanding needs of diagnostic and research endeavors.

Keywords: Immunohistochemistry, antigen retrieval, visualization techniques, multiplex staining, digital pathology

DOI: 10.3233/JCB-230110

Journal: Journal of Cellular Biotechnology, vol. 9, no. 2, pp. 131-138, 2023