Abstract: The signal role in the cellular reactions of gaseous transmitters (NO and H2S) is known. However there are only a few studies on the effect of NO on red blood cell aggregation (RBCA) and deformability (RBCD). However it remains virtually unexplored role of hydrogen sulfide as a signaling molecule in the analysis of RBC microrheology changes. The purpose of the present study was to investigate changes of red blood cell (RBC) microrheological properties under the influence of some gasotransmitter donors, based on the use of several red blood cell models. RBC microrheology was recorded after cell incubation with: 1) NO donor – sodium nitroprusside (SNP, 10, 50, 100 μM); 2) hydrogen sulphide donor – sodium hydrosulfide (NaHS, 10, 50, 100 μM). Cells were incubated for 30 min at 37°C. RBC suspension prepared in drug-free solution was used as a control sample. It was found that an exposure of RBCs both types of the gasotransmitter (GT) donors led to significant positive changes in the RBC microrheological properties. To study the dose-dependent effect of GT donors, RBCs were incubated in a medium with different GT donor concentrations. The results of the study showed that SNP was more effective at a concentration of 100 μM, while NaHS – at a concentration of 50 μM. In the study of the RBC ghost microrheology, it was found that SNP increased their deformability by 7% (p < 0.01), whereas NaHS much more, by 12%. Using a cellular model of RBC age fractions, it was found that GT donors moderately and positively influenced the deformability of all three age populations. However, the old erythrocytes more significantly responded to GTs by the increase in their deformability. Taken together obtained data allow us to conclude that both gasotransmitter donors positively affect RBC microrheology: significantly reduce their aggregation and moderately but statistically significant increase the cell deformability.