The influence of the concentration of thrombin on the dynamic viscoelasticity of clotting blood and fibrinogen-thrombin systems

Article type: Research Article

Authors: Kaibara, M. | Fukada, E.

Affiliations: The Institute of Physical and Chemical Research, Wako-shi, Saitama 351, Japan

Abstract: Measurements of dynamic elastic modulus E′ and loss modulus E″ of clotting blood and fibrinogen solutions were carried out. When the blood is collected into a siliconized polyethylene test tube, the saturated value of E′ is smaller and that of E″ is larger, and the clotting time is longer than those when the blood is collected into a non-siliconized glass tube. In the aqueous solution of fibrinogen added with thrombin, the lower concentration of thrombin yielded the smaller E′ and the larger E″ for the clotted gel and the longer clotting time. As the concentration of thrombin is low, the polymerization of fibrin would be impeded by the presence of partly formed gel and the formation of fibrin network would become incomplete. It is supposed that the siliconization of the surface of tube may influence the rate of production and the concentration of thrombin in the blood.

DOI: 10.3233/BIR-1971-83-404

Journal: Biorheology, vol. 8, no. 3-4, pp. 139-147, 1971