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Shear viscosity and nonlinear behavior of whole blood under large amplitude oscillatory shear
Article type: Research Article
Authors: Sousa, P.C.; | Carneiro, J. | Vaz, R. | Cerejo, A. | Pinho, F.T. | Alves, M.A. | Oliveira, M.S.N.
Affiliations: Departamento de Engenharia Química, CEFT, Faculdade de Engenharia da Universidade do Porto, Porto, Portugal | Departamento de Neurocirurgia, Hospital S. João, Porto, Portugal | Departamento de Engenharia Mecânica, CEFT, Faculdade de Engenharia da Universidade do Porto, Porto, Portugal | James Weir Fluids Laboratory, Department of Mechanical and Aerospace Engineering, University of Strathclyde, Glasgow, UK
Note:  Address for correspondence: Dr. P.C. Sousa, Departamento de Engenharia Química, CEFT, Faculdade de Engenharia da Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal. Tel.: +351 22 508 1404; Fax: +351 22 508 1449; E-mail: [email protected]
Keywords: Whole blood rheology, shear viscosity, anticoagulant effect, large amplitude oscillatory shear (LAOS)
Journal: Biorheology, vol. 50, no. 5-6, pp. 269-282, 2013
Received 22 July 2013
Accepted 25 September 2013
We investigated experimentally the rheological behavior of whole human blood subjected to large amplitude oscillatory shear under strain control to assess its nonlinear viscoelastic response. In these rheological tests, the shear stress response presented higher harmonic contributions, revealing the nonlinear behavior of human blood that is associated with changes in its internal microstructure. For the rheological conditions investigated, intra-cycle strain-stiffening and intra-cycle shear-thinning behavior of the human blood samples were observed and quantified based on the Lissajous–Bowditch plots. The results demonstrated that the dissipative nature of whole blood is more intense than its elastic component. We also assessed the effect of adding EDTA anticoagulant on the shear viscosity of whole blood subjected to steady shear flow. We found that the use of anticoagulant in appropriate concentrations did not influence the shear viscosity and that blood samples without anticoagulant preserved their rheological characteristics approximately for up to 8 minutes before coagulation became significant.