Affiliations: Institute of Mechanics and Biomechanics, Bl.4, Sofia, Bulgaria | Institute of Physical Chemistry, Bulgarian Academy of Sciences, Akad. G. Bonchev Str., Bl.11, 1113 Sofia, Bulgaria
Note: [] Address for correspondence: Nadia Antonova, Institute of Mechanics and Biomechanics, Bulgarian Academy of Sciences, Akad. G. Bonchev Str., Bl.4, 1113 Sofia, Bulgaria. Tel.: +3592 9711399/262, Fax: +3592 8707 498; E‐mail: [email protected].
Abstract: The proposed in vitro model for studying the alveolar surface layer of the lungs enables one to investigate the surface intermolecular forces which influence the stability of the alveolus. The general role for the stability of the alveolus belongs to the phospholipids in the alveolar surfactant and predominantly to their main component dipalmitoylphosphatidylcholine (DPPC). The aim of the study was to investigate the rheological behavior of DPPC and exogenous surfactant preparations used in neonatal clinical practice. Data for the rheological behavior of the solutions of the commercially available surfactants, Infasurf, Exosurf and Survanta, as well as of DPPC (their main phospholipid component) at shear rates from 0.024 to 94.5 s−1 under steady and transient flow conditions at 23°C were obtained. Infasurf and Exosurf showed Newtonian rheological behavior, while Survanta revealed the shear‐thinning behavior of a non‐Newtonian pseudoplastic fluid. The rheological properties of aqueous solutions of DPPC containing 0.14 M NaCl at concentrations from 100 and 630 μg/ml of phospholipid (chosen from the dependence of the probability for bilayer film formation) were studied. Differences observed in the rheological properties of the exogenous surfactants were interpreted on the basis of their composition, the presence of other phospholipid components, certain additives and surfactant proteins, as well as the bulk structures formed from them. The relevance of the results for the delivery of exogenous surfactants and their spreading in replacement therapy is discussed.
Keywords: Phospholipids, lung surfactant, viscosity, bilayer films
Journal: Biorheology, vol. 40, no. 5, pp. 531-543, 2003
