Effect of the presence of hardened erythrocytes on deformation-orientation characteristics of normal erythrocytes in shear flow studied by the spin label method

Article type: Research Article

Authors: Kon, K.^a | O’Bryan, E.R.^b | Kon, H.^a

Affiliations: [a] Laboratory of Chemical Physics, National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases, National Institute of Health, Bethesda, MD 20205, USA | [b] Computer Systems Laboratory, Division of Computer Research and Technology, National Institute of Health, Bethesda, MD 20205, USA

Note: [] Accepted by: Editor R. Skalak

Abstract: The effect of the presence of hardened red blood cells (HRBC) in a mixed suspension on the deformation-orientation characteristics of normal cells in flow is experimentally probed by the electron spin resonance (ESR) spin label method, using a phosphatidylcholine label which does not transfer between the cells. The average deformation-orientation of the normal cells is generally suppressed by the presence of HRBC to different degrees, depending upon the shape and the way the HRBC are prepared. The effects are qualitatively explained by disturbance of laminar shear flow due to the random tumbling of the HRBC.

Keywords: Spin label, Erythrocyte, Deformation-orientation, Cell-cell interaction, Hardened cells

DOI: 10.3233/BIR-1985-22202

Journal: Biorheology, vol. 22, no. 2, pp. 105-117, 1985