The anionic barrier of blood vessel walls and the possible pathologic changes due to the deionization of the barrier and plasma proteins

Issue title: Memorial Issue in Honor of Bun’ichi Tamamushi

Guest editors: Syoten Oka and Eiichi Fukada

Article type: Research Article

Authors: Seno, S. | Tsujii, T. | Ono, T.

Affiliations: Shigei Medical Research Institute, 2117 Yamada, Okayama 701-02, Japan

Note: [] Accepted by: Editor E. Fukada

Abstract: For the purpose to observe the possible vessel wall damage by deionization of the related anionic groups the perfusion test of rat kidney and aorta with cationic iron colloid particles and the injection experiments with albumin-poly-L-lysine complex were carried out. By staining the fixed tissue sections with the cationic iron colloid at pH 7.3 the ionized anionic sites on vessel walls have been demonstrated histochemically. The perfusion test of living tissue with the cationic iron colloid induced the desquamation of the endothelial cells. The perfused colloid particles were adsorbed to the whole endothelial cell surfaces facing to luminal and subliminal sides. Albumin-poly-L-lysine complex injected into vein deposited in the subendothelial spaces of glomerular capillaries through the endothelial cell layer having the well-kept anionic barriers. Discussion was made on possible damage of vessels due to the deionization of anionic groups of endothelial cells and serum proteins by general cationic molecules.

Keywords: anionic barrier, cationic particles, albumin-poly-L-lysine complex, endothelial cell damage

DOI: 10.3233/BIR-1983-20521

Journal: Biorheology, vol. 20, no. 5, pp. 653-662, 1983