Laser Doppler anemometry on individual red blood cells

Article type: Research Article

Authors: Eichhorn, Jean-Louis

Affiliations: Institut de Mécanique des Fluides ERA CNRS 594, Université Louis Pasteur, Strasbourg, France

Note: [] Accepted by: Editor J.C. Healy

Abstract: In order to take measurements of electrical and mechanical characteristics on red blood cells in motion in a hydrodynamic or electric field, a micro laser doppler anemometry apparatus was developed. Because of the small dimensions of the scattering volume of this apparatus, measurements can be taken on individual cells. Two fields of measurement were studied: that of red blood cells in free motion in a hydrodynamic field (speeds of a few mm/s), an that of very low speeds (a few μm/s) the cells moving in a hydrodynamic or an electric field. We can thus obtain simultaneously for each red blood cell: the apparent dimension, the speed, the electrical charge. This methodology gives us multiparametric statistics on individual characteristics of a population or red blood cells.

Keywords: Erythrocyte, Laser anemometry, microscope

DOI: 10.3233/BIR-1982-19504

Journal: Biorheology, vol. 19, no. 5, pp. 621-629, 1982