In vitro study of intracellular IL‐1β production and β1 integrins expression in stimulated chondrocytes – Effect of rhein

Issue title: 2nd International Symposium on Mechanobiology: Cartilage and Chondrocyte. Paris, France, April 2001

Article type: Research Article

Authors: Gigant‐Huselstein, C. | Dumas, D. | Payan, E. | Muller, S. | Bensoussan, D. | Netter, P. | Stoltz, J.F.^;

Affiliations: Mécanique et Ingénierie Cellulaire et Tissulaire, LEMTA UMR INPL CNRS 7563 et IFR 111 Bioingénierie, Faculté de Médecine, 54505 Vandoeuvre‐lès‐Nancy, France | Physiopathologie et Pharmacologie Articulaires – UMR CNRS 7561 et IFR 111 Bioingénierie, Faculté de médecine, 54504 Vandoeuvre‐lès‐Nancy, France

Note: [] Address for correspondence: Jean François Stoltz, Mécanique et Ingénierie Cellulaire et Tissulaire, LEMTA UMR CNRS 7563, Faculté de Médecine ‐ B.P. 184, 54505 Vandoeuvre‐lès‐Nancy Cedex, France. Tel.: +33 03.83.59.26.41; Fax.: +33 03.83.59.26.43; E‐mail: [email protected]‐nancy.fr.

Abstract: The purpose of the present study was to investigate the intracellular IL‐1β production and β1 integrins (α4/β1 and α5/β1) expression on chondrocytes. Chondroytes monolayer (human chondrosarcoma cell line HEM‐C55) were incubated for 12, 24 and 48 hours in the presence of Tumor Necrosis Factor‐α (TNF‐α, Sigma, France) or recombinant human IL‐1α (rh‐IL1α, Becton Dickinson, France). After direct immunolabelling, cells were either analyzed on FACScan flow cytometer (Becton Dickinson, France), or observed under an epi‐fluorescence inverted microscope equipped with the CellScan EPRTM optical scanning acquisition system (IPLab‐Scanalytics, USA). We found that the IL‐1β mean fluorescence intensity in flow cytometry and in 3D microscopy was increased in the presence of TNF‐α or rh‐IL‐1α, and α4/β1 or α5/β1 expression was higher on stimulated cells than on control cells. On the other hand, we have evaluated the in vitro effects of rhein (10−5 M, Negma, France), an active metabolite of diacerein, on the intracellular IL‐1β and β1 integrins expressed by stimulated or no‐stimulated chondrocytes. The results indicated that rhein leads to a reduction of IL‐1β synthesis whereas a weak decrease of β1 integrins receptors expression is observed. From this study, it seems that rhein partially reduce cytokine‐induced intracellular IL‐1β production, and it has a weak action on α4/β1 or α5/β1 receptors.

Journal: Biorheology, vol. 39, no. 1-2, pp. 277-285, 2002