Modulation of energy metabolic enzyme expression in
N-nitrosodiethylamine-mediated hepatocarcinogenesis by Chinese herbs, Huqi
San
Issue title: Integrated Functions of Diet in Anti-Aging and
Cancer Prevention: Papers from the 4th International Niigata Symposium on Diet
and Health, 20–30 November 2008, Niigata, Japan
Affiliations: Pathophysiological Department, Capital Medical
University, Beijing 100069, P.R. China | Beijing Ji Shui Tan Hospital, Beijing 100035, P.R.
China
Note: [] Address for correspondence: Prof. Wang Xuejiang, Department of
pathophysiology, Capital Medical University, No.10 Xitoutiao, You An Men,
Beijing 100069, P.R. China. Tel.: +86 83911434; Fax: +86 83911496; E-mail:
[email protected]
Abstract: Aim: To elucidate the anticancer mechanism of Huqi San by assessing
the expression of G-6-Pase, SDH, ATPase and AFP in
N-nitrosodiethylamine-mediated hepatocarcinogenesis in rats. Methods: A Solt-Farber two-step test model of hepatocarcino genesis
was established by diethylnitrosamine (DEN) and 2-acetylaminofluorene (AAF) in
rats to investigate the modifying effects of expression of 6-glucosephosphatase
(G-6-Pase), succinodehydrogenase (SDH), adenosine triphosphatase (ATPase) in
N-nitrosodiethylamine-mediated hepatocarcinogenesis. Hu Qisan compounded by
eight medicinal herbs was prepared in glycoprival granules with wich 0.38 g
crude herbs/mL solution was prepared for administration.
γ-Glutamy-transpeptidase (γ-GT), G-6-Pase, SDH and ATPase were
immunohistochemically determined. The activity of alpha-fetoglobulin (AFP) in
the livers was measured with Immunofluorescence. Results: Huqi San treated rats showed significant decrease in areas
of γ-GT positive foci (P< 0.001). On the other hand, the expression of
G-6-Pase, SDH and ATPase has obviously altered in Huqi San treated group. The
activity of AFP also significantly decreased after the treatment with Huqi San
(8 g/kg body weight or 4 g/kg body weight) or total alkali of mistletoe (0.12
g/kg body weight). Conclusions: Huqi San can obviously increase these activities of
G-6-Pase, SDH and ATPase, and at the same time significantly decrease the
expression of γ-GT and AFP. Therefore, it can obstruct or inhibit the
rat's liver preneolastic lesion induced by DEN.
