Affiliations: Molecular Medicine Research Center, Chang Gung
University, Kwei-san, Tao-yuan, Taiwan | Graduate Institute of Medical Biotechnology and
Department of Medical Biotechnology and Laboratory Science, Chang Gung
University, Kwei-san, Tao-yuan, Taiwan | Department of Clinical Pathology, Chang Gung Memorial
Hospital, Kwei-san, Tao-yuan, Taiwan | Department of Life Science, Chang Gung University,
Kwei-san, Tao-yuan, Taiwan
Note: [] Address for correspondence: Dr. Ming-Shi Shiao, Department of
Life Science, Institute of Biomedical Science, Chang Gung University, No. 259,
Wen-Hwa 1st Rd., Kwei-san, Tao-yuan, Taiwan. Tel.: +886 3 2118800 ext 3495;
Fax: +886 3 2118700; E-mail: [email protected]
Abstract: Recent advances in the use of liquid chromatography-mass
spectrometry for the study of metabolomics are reviewed. Sample preparations of
biofluids and practical aspects of ultra-high pressure liquid chromatography
are discussed. Applicability of different kinds of mass spectrometers for
metabolite profiling is described. New tools-ion mobility spectroscopy and
automated chip-based nanoelectrospray system with potentials to be applied in
the metabolomics analysis are described.
Keywords: Metabolomics, metabolite profiling, UHPLC, LC-MS, ion mobility spectroscopy
Journal: BioFactors, vol. 34, no. 2, pp. 159-169, 2008
