Affiliations: The Center for Traditional Microorganism Resources,
Keimyung University, Daegu, Korea
Note: [] Address for correspondence: Professor In-Seon Lee, The Center
for Traditional Microorganism Resources, Keimyung University, 1000,
Shindang-dong, Dalseo-gu, Daegu, 704-701, Korea. Tel.: +82 53 580 5538; Fax:
+82 53 580 5538; E-mail: [email protected]
Abstract: The antioxidant effects of Artemisia capillaris fractions against
reactive oxygen species (ROS) were evaluated by measuring scavenging activities
against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide
(O_{2}^{-}), hydroxyl (HO·) and
nitric oxide (NO·) radical. Among five solvent fractions, ethyl acetate
fraction showed the highest total polyphenol and total flavonoid contents as
648.75 and 89.09 μg/mg, respectively. Also, the ethyl acetate fraction
showed the highest scavenging activity; the 50% inhibitory concentration
(IC_{50}, μg/mg) value for DPPH,
O_{2}^{-}, HO· and NO· radical scavenging were
4.76, 31.54, 69.34 and 74.63, respectively. Additionally, the highest
inhibition of rat liver microsomal lipid peroxidation was observed by ethyl
acetate fraction. Except for free radical-mediated protein damage, ethyl
acetate fraction showed the highest scavenging activity. The effect of
Artemisia capillaris fractions on cell viability and DNA damage induced by
H_{2}O_{2} in Raw 264.7 cell were also
evaluated by MTT and comet assay, respectively. The protective effect of ethyl
acetate fraction, as indicated by cell viability increasing 71% and DNA
breakage decreasing 51% as compared with
H_{2}O_{2}-treated positive control.
These results suggest that ethyl acetate fraction possess significant ROS
scavenging and protective effect against oxidative DNA damage.
Keywords: Antioxidant, Artemisia capillaris, reactive oxygen species, DNA damage
Journal: BioFactors, vol. 31, no. 1, pp. 43-53, 2007
