Affiliations: Research Center, Natural F&P Co., Ltd,
Chuncheon, South Korea | Department of Anatomy and Cell Biology, College of
Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National
University, Seoul, South Korea | Department of Anatomy and Neurobiology, College of
Medicine, Hallym University, Chuncheon, South Korea | Department of Anatomy, College of Veterinary Medicine
and Institute of Animal Science, Gyeongsang National University, Jinju, South
Korea
Note: [] Address for correspondence: Moo-Ho Won, Department of Anatomy
and Neurobiology, College of Medicine, Hallym University, Chuncheon 200-702,
South Korea. Fax: +82 33 256 1614; E-mail: [email protected]
Abstract: Coenzyme Q_{10} (CoQ_{10}) is
a naturally occurring antioxidant and a prominent component of mitochondrial
electron transport chain. In the present study, we investigated the effect of
CoQ_{10} nanoparticle against photoaging using
immunohistochemistry and Western blot analysis in the hairless mouse skin
induced by ultraviolet B (UVB) irradiation (300 mJ/cm^{2},
3 min/day for 21 days). In the UVB-irradiated distilled water (DW)-treated
group, manganese superoxide dismutase (SOD2) and glutathione peroxidase (GPx)
immunoreactivity and their protein levels in the skin were significantly lower
than those in the control group. However, SOD2 and GPx immunoreactivity and
their protein levels in the skin of the UVB-irradiated
CoQ_{10}-treated group were higher than those in the
UVB-irradiated DW-treated group. GPx activity in the skin in the UVB-irradiated
DW-treated group significantly decreased compared to that in the control group;
whereas GPx activity in the UVB-irradiated CoQ_{10}-treated
group was similar to that in the control group. These results suggest that
CoQ_{10} strongly inhibits oxidative stress in the skin
induced by UVB via increasing SOD2 and GPx.
