Affiliations: Department of Biophysics, Belarus State University,
Minsk, Belarus | Pediatric Clinic of Johann Wolfgang Goethe University,
Theodor-Stern-Kai 7, 60596 Frankfurt am Main, Germany
Note: [] Address for correspondence: A.A. Krjukov, Department of
Biophysics, Belarus State University, Ave. Independence (Nesavisimosty) 4,
220050 Minsk, Belarus. Tel.: +375 017 209 50 81; Fax: +375 017 209 54 45;
E-mail: [email protected]; [email protected]
Abstract: In this work the influence of
H_{2}O_{2} on the ability of human blood
monocytes to generate ROS upon stimulation of cells by adhesion to glass
surface and fMLP was studied using the luminol-dependent chemiluminescence
(LDCL) method. Pretreatment of cells with
H_{2}O_{2} increased the adhesiveness of
monocytes and ROS generation. Superoxide generation by cells in response to
fMLP depended on the duration of pretreatment and the concentration of
H_{2}O_{2}. The stimulatory effect on
fMLP-induced LDCL of cells further depended on the Ca^{2+}
concentration in the medium and on the activities of phospholipase
A_{2}, cyclooxygenases, and Mek1/2.
