Affiliations: UMR 1089-Xénobiotiques, INRA/ENVT, BP 3, 31931,
Toulouse Cedex 9, France | Fédération de Biochimie, Hôpital
Édouard Herriot, 69437, EA 3090, UCBLI, Lyon, France
Note: [] Address for correspondence: F. Guéraud, UMR
1089-Xénobiotiques, INRA/ENVT, BP 3, 31931, Toulouse Cedex 9, France.
Tel.: +33 561 285 383; Fax: +33 561 285 244; E-mail: [email protected]
Abstract: The objective of our study was to compare the information obtained
through the use of three different urinary biomarkers of lipoperoxidation
during the time course of a bromotrichloromethane (BrCCl_3)
induced oxidative stress in rats. These biomarkers were malondialdehyde (MDA)
measured by LC/MS after derivatization, the isoprostane
8-iso-PGF_{2α} measured by enzyme immunoassay and
1,4-dihydroxynonene mercapturic acid (DHN-MA), the major 4-hydroxynonenal
urinary metabolite [1], measured by LC-MS. Male Wistar rats received a
single dose of 100 μL/kg BrCCl_3
per os and lipid peroxidation was estimated every day for a 4-day-period after
treatment. MDA, 8-iso-PGF_{2α} and DHN-MA significantly
increased in response to BrCCl_3 treatment for this period
of time, and DHN-MA showed the main increase during the 24–48 h period after
treatment.
