Affiliations: School of Food and Nutritional Sciences | Graduate School of Nutritional and Environmental
Sciences, University of Shizuoka, Yada 52-1, Shizuoka 422-8526, Japan
Note: [] School of Food and Nutritional Sciences, University of Shizuoka,
Yada 52-1, Shizuoka 422-8526, Japan. Tel. and Fax: +81 54 264 5528; E-mail:
[email protected]
Abstract: Luteolin has been shown to possess potent antioxidant and
anti-inflammatory/anti-allergic activities. In order to evaluate a
chemopreventive role of luteolin in inflammatory responses involved in the
pathogenesis of atherosclerosis and cancer etc., the metabolic fate of luteolin
in rats and humans was investigated by HPLC analysis, and its effect on cell
surface expression of adhesion molecules in human umbilical vein endothelial
cells(HUVECs) was examined by ELISA. Luteolin monoglucuronide, which was a main
metabolite, and free luteolin were detected in rat plasma and human serum.
Luteolin monoglucuronide was hydrolyzed to free luteolin by
\beta-glucuronidase released from neutrophils stimulated
with Ionomycin and Cytocharasine B. Luteolin suppressed the
TNF-\alpha induced ICAM-1 expression significantly. Among
nine flavonoids (40 \muM) examined, chrysin, apigenine,
quercetin and galangin also demonstrated suppressive effct on it. These results
suggest the posssibility that deconjugation of luteolin monoglucuronide occurs
and that free luteolin showed functional acyivities such as suppression of
TNF-\alpha induced ICAM-1 expression at inflammation
site.
