Therapeutic evaluation of titanium dioxide nanoparticles based herbal dental varnish derived from rosemary and ginger extracts:A comprehensive investigation into anti-inflammatory and antioxidant properties

Article type: Research Article

Authors: Maheshwaran, Bipin^a | Chokkatt, Jerry Joe^a | Shenoy, Amrutha^a | Ganapathy, Dhanraj^a | Yadalam, Pradeep Kumar^b | Marrapodi, Maria Maddalena^{c; *} | Cicciù, Marco^d | Minervini, Giuseppe^{e; f}

Affiliations: [a] Department of Prosthodontics and Implantology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India | [b] Department of Periodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India | [c] Department of Woman, Child and General and Specialist Surgery, University of Campania “Luigi Vanvitelli”, Naples, Italy | [d] Department of Biomedical and Surgical and Biomedical Sciences, Catania University, Catania, Italy | [e] Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, Tamil Nadu, India | [f] Multidisciplinary Department of Medical-Surgical and Dental Specialties, University of Campania Luigi Vanvitelli, Naples, Italy

Correspondence: [*] Corresponding author: Maria Maddalena Marrapodi, Department of Woman, Child and General and Specialist Surgery, University of Campania “Luigi Vanvitelli”, Naples, Italy. E-mail: [email protected].

Abstract: BACKGROUND: Titanium nanoparticles (NPs) offer promising applications in the treatment and prevention of inflammatory disorders due to their unique physicochemical characteristics. However, additional research is necessary to attain a thorough comprehension and validate the efficacy of this approach in dental practice. OBJECTIVE: This study scrutinizes the anti-inflammatory properties of a dental varnish infused with ginger and rosemary extracts mediated by titanium dioxide (TiO2) nanoparticles. METHODS: A herbal dental varnish was formulated by integrating ginger and rosemary extracts with titanium dioxide nanoparticles at concentrations of 10, 20, 30, 40, and 50 μL. Anti-inflammatory properties were assessed through Bovine Serum Albumin denaturation and membrane stabilization assays, comparing results with a control group. RESULTS: The results reveal concentration-dependent antioxidant and anti-inflammatory properties in the test group when compared to the control group. The BSA assay corroborates increased percent inhibition with rising titanium dioxide nanoparticle concentrations. In line with existing literature, titanium dioxide nanoparticles enhance dental material properties. CONCLUSION: The bioactive compounds in ginger and rosemary, such as phenolic compounds and terpenes, contribute to anti-inflammatory and antioxidant effects of the varnish. Additionally, the therapeutic potential of titanium dioxide nanoparticles in addressing inflammatory diseases underscores their significance in this formulation.

Keywords: Preventive dentistry, nanoparticles, titanium dioxide, green synthesis, antioxidant

DOI: 10.3233/THC-231994

Journal: Technology and Health Care, vol. 32, no. 4, pp. 2783-2792, 2024