Affiliations: [a] Department of Cell Biology and Medical Genetics, Yanbian University Medical College, Yanji, Jilin, China | [b] Department of Endocrinology, Affiliated Hospital of Yanbian University, Yanji, Jilin, China
Correspondence:
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Corresponding author: Lian-Shan Piao, Department of Endocrinology, Affiliated Hospital of Yanbian University, No. 1327 of Juzi Street, Yanji 133000, Jilin, China. Tel.: +86 15526770341; E-mail: [email protected].
Abstract: OBJECTIVE: To investigate the association of single nucleotide polymorphisms (SNPs) of soluble low-density lipoprotein receptor 11 (sLR11) genes with type-2 diabetes mellitus (T2DM) and carotid atherosclerosis (CAS) in Korean and Han nationalities in the Yanbian area. METHODS: 530 T2DM patients were divided into two groups according to the intima-media thickness (IMT) of the carotid artery: CAS group (n= 256, T2DM patients with carotid artery IMT ⩾ 1.0 mm and plaque) and non-CAS group (NCAS group, n= 274, T2DM patients with carotid IMT < 1.0 mm). IMT and plaque were measured by color Doppler ultrasound. SNP typing and sequencing were performed by PCR-LDR. RESULTS: 1. Allele frequency and genotype frequency distribution results: Differences in genotype and allele frequency distribution between the CAS and NGT groups, the NCAS and NGT groups, and the CAS and NCAS groups were not statistically significant (P> 0.05). The dominant and recessive modes were analyzed, but the difference in genotype frequency among these three groups was not statistically significant (P> 0.05). Differences in genotype frequency distribution between Korean and Han populations in all three groups were not statistically significant (P> 0.05). 2. Correlation analysis with clinical indicators: LDL-C levels in TT and AT patients in the CAS group were significantly higher than those in AA patients (P> 0.05), representing the dominant mode of inheritance.. CONCLUSION: This study is the first to determine that the sLR11 gene rs3824968 polymorphic of factor T may increase the risk of CAS in T2DM patients by regulating the concentration of LDL-C, showing the dominant mode of inheritance.
Keywords: sLR11, gene polymorphism, type 2 diabetes mellitus, carotid intima media thickness
