Affiliations: [a] Department of Automatic Control Engineering, Feng-Chia University, Taichung, Taiwan | [b] Ph.D. Program of Electrical and Communications Engineering, Feng-Chia University, Taichung, Taiwan
Correspondence:
[*]
Corresponding author: Hsing-Cheng Chang, Department of Automatic Control Engineering, Feng-Chia University, 100, Wen-hwa Rd, Sea-twen, Taichung, Taiwan. E-mail:[email protected]
Abstract: In this study, an automatic microfluidic
fluorescence-array measurement system is developed to detect the
concentration of organic phosphate based on the luminol-hydrogen peroxide
catalytic fluorescent mechanism. Not only sample quantity and cost can be
reduced, but also detection time, accuracy and precision can be improved in
the system. The system is composed of a CCD image module, a stepper motor
with driver, a microfluidic fluorescence array, a background light
elimination module, and a dynamic image-analyzed interface. The pesticides
of chlorpyrifos and fenitrothion of organic phosphate are chosen as
experimental samples. Only a 2.5 μ l quantity of sample is required to
have a fast response time of 1.4 second. Experimental results show that the
sensitivities of chlorpyrifos and fenitrothion are 1.88 V/ppm in the range
of 0.166 ∼ 10 ppm with averaged error of 1.66% and 0.32 V/ppm in
the range of 0.03 ∼ 10 ppm with averaged error of 1.68%
respectively. The organophosphorus effective detection range of the
developed system covers the legal prescription for pesticide residues.
Keywords: Organophosphorus measurement, centrifugal microfluidic, fluorescence-array, chlorpyrifos and fenitrothion
